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L1613-BC 1 M HEPES Buffer (50x)

A 1 M solution of HEPES that provides good buffering in the pH range of 7.2 to 7.6.

L1613-BC
  
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      Overview

      Replacement Information
      Description
      OverviewHEPES (hydroxyethyl-piperazineethane-sulfonic acid buffer) is an organic buffer and very stable. In the physiological area HEPES has an excellent buffer capacity in a pH range of 7.2 to 7.6. HEPES is apt for all cell culture systems with a high proliferation and cell density. On HEPES buffered cell culture media, the incubation in a CO2 incubator is not necessary.

      Specifications:

      Sterility: Pass
      pH: 7.35-7.55
      Osmolality: 1.65-2.00 Osm/kg H20
      Particles/ml (>10µM): < 25/ml
      Particles/ml (>25µM): < 3/ml
      Color: Colorless
      Appearance: Clear

      How to use HEPES buffer:

      • dissolve powder and adjust pH at 7.2 with sodium hydroxide
      • add 10-20 ml of HEPES buffer per litre of medium (for very delicate cell cultures, use only 10 ml)
      • a concentration of 20 mM HEPES requires 20 ml/l medium, for 25 mM use 25 ml/l
      • if NaHCO3 is required, only add 10%
      Catalogue NumberL1613-BC
      Brand Family Calbiochem®
      References
      Product Information
      Quality LevelMQ300
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Storage +2°C to +25°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      L1613-BC 0

      Documentation