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194437 ABTS™ Chromophore, Diammonium Salt

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      OverviewContains 20 tablets. Each tablet contains 5 mg and makes 5 ml of ABTS™ Chromophore working substrate for ELISA.
      Catalogue Number194437
      Brand Family Calbiochem®
      Product Information
      CAS number30931-67-0
      Hill FormulaC₁₈H₁₈N₄O₆S₄ · (NH₃)₂
      Chemical formulaC₁₈H₁₈N₄O₆S₄ · (NH₃)₂
      Quality LevelMQ100
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/37/38

      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Irritant
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsUnstable in solution; reconstitute just prior to use and discard any unused reconstituted substrate.
      Packaging Information
      Transport Information
      Supplemental Information


      ABTS™ Chromophore, Diammonium Salt SDS


      Safety Data Sheet (SDS) 

      ABTS™ Chromophore, Diammonium Salt Certificates of Analysis

      TitleLot Number
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-March-2009 JSW
      DescriptionUseful as a substrate for ELISA; acts as a hydrogen donor in peroxidase reactions. Radical cation has an absorption maximum at 414 nm.
      Recommended reaction conditions
      For Use as ELISA Substrate Note: Each tablet contains 5 mg and yields 5 ml ABTS® working substrate for ELISA when reconstituted as outlined below. 1. Dissolve ABTS at a final conc. of 1 mg/ml in 0.1 M sodium citrate buffer at pH 4.2. 2. Add 1.8 µl 30% H2O2 to 6 ml of the 1 mg/ml ABTS solution above (or 3 µl 3% H2O2 to 1 ml of the ABTS solution). 3. Add 1 ng HRP-conjugated antibody or HRP to 1 ml of the ABTS solution containing H2O2. 4. Incubate for 30 min to 1 h at 37°C (room temp is also OK, but the reaction is faster at 37°C). 5. Stop the reaction at the end of incubation with 1 ml (i.e. equal volume) of stop solution (0.625 M oxalic acid) and read the absorbance at 405 nm.
      CAS number30931-67-0
      Chemical formulaC₁₈H₁₈N₄O₆S₄ · (NH₃)₂
      Solubility0.1 M sodium citrate buffer, pH 4.2 (1 mg/ml)
      Storage +2°C to +8°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsUnstable in solution; reconstitute just prior to use and discard any unused reconstituted substrate.
      Toxicity Irritant