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CB1024 Anti-ACTN4 Mouse mAb (4D10)

Anti-ACTN4, mouse monoclonal, clone 4D10, recognizes the ~90-100 kDa ACTN4 in PC-12, HeLa, Raw 264.7, MCF-7, NIH3T3 cells & in human testes. It is validated for ELISA, WB, ICC, and paraffin sections.

Anti-ACTN4 Mouse mAb (4D10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Actinin α4

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CB1024
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Pricing & Availability

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CB1024-100UG
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      Description
      OverviewRecognizes the ~90-100 kDa ACTN4 protein in PC-12, HeLa, Raw 264.7, MCF-7, and NIH3T3 cells and human testes tissue.
      Catalogue NumberCB1024
      Brand Family Calbiochem®
      SynonymsAnti-Actinin α4
      Application Data
      Detection of rat ACTN4 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of Human ACTN4 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse ACTN4 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of Human ACTN4 by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Priamry antibody: Anti-ACTN4 Mouse mAb (AD10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human ACTN4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (3 µg/ml). Detection: colorimetric (DAB).

      Detection of human ACTN4 by immunocytochemistry. Sample: Paraformaldahyde treated HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (20 µg/ml). Detection: fluorescence).
      References
      ReferencesYamada, S., et al. 2010. Int. J. Oral Maxillofac. Surg. 39, 61.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlPC-12 cells, HeLa cells, Raw 264.7 cells, MCF-7 cells, NIH3T3 cells, Human testes tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesELISA (see coments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (20 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 592-702 of human ACTN4, espressed as a GST fusion protein
      Clone4D10
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CB1024-100UG 04055977228694

      Documentation

      Anti-ACTN4 Mouse mAb (4D10) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-ACTN4 Mouse mAb (4D10) Certificates of Analysis

      TitleLot Number
      CB1024

      References

      Reference overview
      Yamada, S., et al. 2010. Int. J. Oral Maxillofac. Surg. 39, 61.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-October-2010 RFH
      SynonymsAnti-Actinin α4
      ApplicationELISA (see coments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (20 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of rat ACTN4 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of Human ACTN4 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse ACTN4 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of Human ACTN4 by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Priamry antibody: Anti-ACTN4 Mouse mAb (AD10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human ACTN4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (3 µg/ml). Detection: colorimetric (DAB).

      Detection of human ACTN4 by immunocytochemistry. Sample: Paraformaldahyde treated HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (20 µg/ml). Detection: fluorescence).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~90-100 kDa ACTN4 protein.
      BackgroundAlpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 592-702 of human ACTN4, espressed as a GST fusion protein
      Clone4D10
      IsotypeIgG2a
      Specieshuman, mouse, rat
      Positive controlPC-12 cells, HeLa cells, Raw 264.7 cells, MCF-7 cells, NIH3T3 cells, Human testes tissue
      Negative control293T cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesYamada, S., et al. 2010. Int. J. Oral Maxillofac. Surg. 39, 61.