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MAB3785 Anti-APC Antibody, NT, clone Ali 12.28

Use Anti-APC Antibody, N-terminus, clone Ali 12.28 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB, IHC to detect APC also known as Adenomatous Polyposis Coli Protein.

Anti-APC Antibody, NT, clone Ali 12.28 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: APC, DP2.5, FAP, GS, DP3, DP2, FPC UniProt Number: P25054
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MAB3785
100 µg  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, IP, WB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3785
      ReplacesAB1268
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-APC Antibody, NT, clone Ali 12.28
      Alternate Names
      • Adenomatous Polyposis Coli Protein
      Background InformationThe adenomatous polyposis coli tumor suppressor gene is mutated (often deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and over 80% of colorectal cancers. Ali 12-28 can be used for APC expression and detection of APC mutations.
      References
      Product Information
      FormatPurified
      Control
      • POSITIVE CONTROL: SW480, LoVo, DLD1, and SW837 cell lines
      PresentationPurified immunoglobulin. Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-APC Antibody, N-terminus, clone Ali 12.28 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB, IHC to detect APC also known as Adenomatous Polyposis Coli Protein.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern blot: 4 μg/mL

      Immunohistochemistry (Acetone Fixed, Frozen)

      ELISA

      Immunoprecipitation

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenN-terminal APC-MBP fusion protein (first 1.3 kb of APC which includes the first 433 aa and the first 9 exons of the gene).
      EpitopeN-terminus
      CloneAli 12.28
      HostMouse
      SpecificityRecognizes human APC, molecular weight ~310 kDa, as well as C-terminal truncation mutants containing the epitope mapped between amino acids 135 to 422.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a tumor suppressor protein that includes among its many intracellular functions one of nuclear export. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.
      Gene Symbol
      • APC
      • DP2.5
      • FAP
      • GS
      • DP3
      • DP2
      • FPC
      Non-Reactive Species
      • Mouse
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P25054 # Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling. APC activity is correlated with its phosphorylation state.
      SIZE: 2843 amino acids; 311646 Da
      SUBUNIT: Forms homooligomers. Interacts with DIAPH1 and DIAPH2 (By similarity). Interacts with PDZ domains of DLG1 and DLG3. Associates with catenins. Binds axin. Interacts with the N- terminus of ARHGEF4, and the C-terminus of MAPRE1, MAPRE2 and MAPRE3. Found in a complex consisting of ARHGEF4, APC and CTNNB1.
      TISSUE SPECIFICITY: Expressed in a variety of tissues.
      PTM: Phosphorylated by GSK3B.
      DISEASE: SwissProt: P25054 # Defects in APC are a cause of familial adenomatous polyposis (FAP) [MIM:175100]; which includes also Gardner syndrome (GS). FAP and GS contribute to tumor development in patients with uninherited forms of colorectal cancer. FAP is characterized by adenomatous polyps of the colon and rectum, but also of upper gastrointestinal tract (ampullary, duodenal and gastric adenomas). This is a viciously premalignant disease with one or more polyps progressing through dysplasia to malignancy in untreated gene carriers with a median age at diagnosis of 40 years. & APC mutations have led to some interesting observations. (1) the great majority of the mutations found to date would result in truncation of the APC product. (2) almost all the mutations have occurred within the first half of the coding sequence, and somatic mutations in colorectal tumors are further clustered in a particular region, called MCR (mutation cluster region). (3) most identified point mutations in the APC gene are transitions from cytosine to other nucleotides. (4) the location of germline mutations tends to correlate with the number of colorectal polyps in FAP patients. Inactivation of both alleles of the APC gene seems to be required as an early event to develop most adenomas and carcinomas in the colon and rectum as well as some of those in the stomach. & Defects in APC are a cause of hereditary desmoid disease (HDD) [MIM:135290]; also called familial infiltrative fibromatosis (FIF). It is an autosomal dominant trait with 100% penetrance and possible variable expression among affected relatives. HDD patients show multifocal fibromatosis of the paraspinal muscles, breast, occiput, arms, lower ribs, abdominal wall, and mesentery. Desmoid tumors appears also as a complication of familial adenomatous polyposis. & Defects in APC are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. Although the majority of medulloblastomas occur sporadically, some manifest within familial cancer syndromes such as Turcot syndrome and basal cell nevus syndrome (Gorlin syndrome). & Defects in APC are a cause of Turcot syndrome [MIM:276300]. Turcot syndrome is an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas. Skin features include sebaceous cysts, hyperpigmented and cafe au lait spots.
      SIMILARITY: SwissProt: P25054 ## Contains 7 ARM repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB3785 04053252666544

      Documentation

      Anti-APC Antibody, NT, clone Ali 12.28 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-APC Antibody, NT, clone Ali 12.28 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN APC (N-TERMINAL) 2842904
      MOUSE ANTI-HUMAN APC (N-TERMINAL) MONOCLONAL ANTIBODY - 2398153 2398153
      MOUSE ANTI-HUMAN APC (N-TERMINAL) - 2496257 2496257
      MOUSE ANTI-HUMAN APC (N-TERMINAL) - 3269450 3269450
      MOUSE ANTI-HUMAN APC (N-TERMINAL) - 3389032 3389032
      MOUSE ANTI-HUMAN APC (N-TERMINAL) - 3854594 3854594
      MOUSE ANTI-HUMAN APC (N-TERMINAL) - 3884776 3884776
      MOUSE ANTI-HUMAN APC (N-TERMINAL) - 3929939 3929939
      MOUSE ANTI-HUMAN APC (N-TERMINAL) - 4248370 4248370
      MOUSE ANTI-HUMAN APC (N-TERMINAL) -3166108 3166108
      Show more

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies