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PC317L Anti-Angiogenin Goat pAb

Anti-Angiogenin Goat pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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PC317L
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HGtPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes angiogenin.

    • Antibody Target Gene Symbol: ANG
    • Target Synonym: AI385586, ALS9, Ang1, Ang2, ANGIOGENIN, ANGIOGENIN 2, BRB, BRN, HEL168, MGC140149, MGC22466, MGC71966, RNASE4, RNASE5, Rnase5a
    • Entrez Gene Name: angiogenin, ribonuclease, RNase A family, 5
    • Hu Entrez ID: 283
    • Mu Entrez ID: 11727
    • Rat Entrez ID: 305843, 497229

      		•
      Catalogue NumberPC317L
      Brand Family Calbiochem®
      References
      ReferencesShapiro, R. 1998. Biochemistry 37, 6847.
      Soncin, F., et al. 1997. Biochem. Biophys. Res. Commun. 236 604.
      Vallee, B.L. and Riordan, J.F. 1997. Cell Mol. Life Sci. 53 803.
      Moenner, M., et al. 1994. Eur. J. Biochem. 226 483.
      Hu, G.F., et al. 1993. PNAS. 90 1217.
      Moroianu, J., et al. 1993. PNAS. 90 3815.
      Soncin, F. 1992. PNAS. 89 2232.
      Lee, F.S. and Vallee, B.L. 1989. Biochem. Biophys. Res. Commun. 161 121.
      Kurachi, K., et al. 1985. Biochemistry 24, 5494.
      Product Information
      FormLyophilized
      FormulationLyophilized from 20 mM ammonium bicarbonate, PBS, 100 µg FAF-BSA.
      Positive controlHT-29 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Neutralization Studies
      Application NotesELISA (0.5-1 µg/ml)
      Immunoblotting (1-2 µg/ml)
      Neutralization Studies (EC₅₀= 100-150 µg/µg angiogenin, see comments)
      Application CommentsThis antibody has been selected for its ability to neutralize the biological activity of human recombinant angiogenin. The activity of human recombinant angiogenin was measured based upon its ribonucleolytic activity toward yeast tRNA. Using the assay conditions described, 1 µg human recombinant angiogenin produces an absorbance change at 260 nm of ~2.0-3.0. The suggested antibody neutralization concentration required to yield 50% of the activity due to 1 µg of human recombinant angiogenin is approximately 100-150 µg of the antibody. The detection limit for human recombinant angiogenin by immunoblotting is ~5 ng/lane under reducing and non-reducing conditions. The detection limit for human recombinant angiogenin by ELISA is ~0.3 ng/well. This antibody exhibits no cross-reactivity with other cytokines when tested in ELISA. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human angiogenin
      ImmunogenHuman
      HostGoat
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 1 mg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC317L 0

      Documentation

      Anti-Angiogenin Goat pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Angiogenin Goat pAb Certificates of Analysis

      TitleLot Number
      PC317L

      References

      Reference overview
      Shapiro, R. 1998. Biochemistry 37, 6847.
      Soncin, F., et al. 1997. Biochem. Biophys. Res. Commun. 236 604.
      Vallee, B.L. and Riordan, J.F. 1997. Cell Mol. Life Sci. 53 803.
      Moenner, M., et al. 1994. Eur. J. Biochem. 226 483.
      Hu, G.F., et al. 1993. PNAS. 90 1217.
      Moroianu, J., et al. 1993. PNAS. 90 3815.
      Soncin, F. 1992. PNAS. 89 2232.
      Lee, F.S. and Vallee, B.L. 1989. Biochem. Biophys. Res. Commun. 161 121.
      Kurachi, K., et al. 1985. Biochemistry 24, 5494.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-October-2007 RFH
      ApplicationELISA (0.5-1 µg/ml)
      Immunoblotting (1-2 µg/ml)
      Neutralization Studies (EC₅₀= 100-150 µg/µg angiogenin, see comments)
      DescriptionImmunoaffinity purified goat polyclonal antibody. Recognizes the ~14 kDa angiogenin protein.
      BackgroundAngiogenin was initially isolated from culture medium conditioned by human colon adenocarcinoma cells (HT-29 cells) as a ~14 kDa protein homologous to pancreatic ribonuclease A. Angiogenin is a potent inducer of blood vessel formation whose enzymatic and three-dimensional relationship to RNAse A is critical for its biological function. It exerts ribonucleolytic activity that is several magnitudes lower than that of RNAse A but nonetheless essential for angiogenesis since inhibition of RNAse activity will also abolish angiogenesis. Internalization and translocation to the nucleolus are also required for activity. Angiogenin binds to high affinity endothelial cell receptors and with lower affinity to extracellular matrix components. An angiogenin binding protein (~42 kDa) previously isolated from the cell surfaces of endothelial cells has been shown to be a member of the actin family by peptide mapping and partial amino acid sequencing. It has also been shown in vitro that angiogenin absorbed on plastic will support the cell adhesion of both endothelial cells and fibroblasts. Angiogenin is differentially produced by anchorage-dependent growing cells including vascular endothelial cells, aortic smooth muscle cells, fibroblasts and tumor cells.
      HostGoat
      Immunogen speciesHuman
      Immunogenrecombinant, human angiogenin
      IsotypeIgG
      Specieshuman
      Positive controlHT-29 cells
      FormLyophilized
      FormulationLyophilized from 20 mM ammonium bicarbonate, PBS, 100 µg FAF-BSA.
      PreservativeNone
      CommentsThis antibody has been selected for its ability to neutralize the biological activity of human recombinant angiogenin. The activity of human recombinant angiogenin was measured based upon its ribonucleolytic activity toward yeast tRNA. Using the assay conditions described, 1 µg human recombinant angiogenin produces an absorbance change at 260 nm of ~2.0-3.0. The suggested antibody neutralization concentration required to yield 50% of the activity due to 1 µg of human recombinant angiogenin is approximately 100-150 µg of the antibody. The detection limit for human recombinant angiogenin by immunoblotting is ~5 ng/lane under reducing and non-reducing conditions. The detection limit for human recombinant angiogenin by ELISA is ~0.3 ng/well. This antibody exhibits no cross-reactivity with other cytokines when tested in ELISA. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 1 mg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesShapiro, R. 1998. Biochemistry 37, 6847.
      Soncin, F., et al. 1997. Biochem. Biophys. Res. Commun. 236 604.
      Vallee, B.L. and Riordan, J.F. 1997. Cell Mol. Life Sci. 53 803.
      Moenner, M., et al. 1994. Eur. J. Biochem. 226 483.
      Hu, G.F., et al. 1993. PNAS. 90 1217.
      Moroianu, J., et al. 1993. PNAS. 90 3815.
      Soncin, F. 1992. PNAS. 89 2232.
      Lee, F.S. and Vallee, B.L. 1989. Biochem. Biophys. Res. Commun. 161 121.
      Kurachi, K., et al. 1985. Biochemistry 24, 5494.