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AP1031 Anti-Apollon (4775-4829) Rabbit pAb

Anti-Apollon (4775-4829) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-BIRC6, Anti-Baculoviral IAP repeat-containing 6, Anti-BRUCE, Anti-KIAA1289

AP1031
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~500 kDa Apollon protein in HeLa cells.
      Catalogue NumberAP1031
      Brand Family Calbiochem®
      SynonymsAnti-BIRC6, Anti-Baculoviral IAP repeat-containing 6, Anti-BRUCE, Anti-KIAA1289
      Application Data
      Detection of human Apollon by immunoprecipitation and immunoblotting. Samples: Whole cell lysate (20 μg, lane 1, 50 μg, lane 2) from HeLa cells or S100 extract (20 μg, lane 3, 50 μg, lane 4) from HeLa cells. Antibody for IP: Anti-Apollon Rabbit pAb (Cat. No. AP1031) (2 μg/mg lysate protein). Antibody for IB: Anti-Apollon Rabbit pAb (Cat. No. AP1031) (1 μg/ml). Detection: chemiluminescence.
      References
      ReferencesQiu, X.B., et al. 2005. J. Biol. Chem. 280, 174.
      Ren, J., et al. 2005. Proc. Natl. Acad. Sci. USA 102, 565.
      Hao, Y., et al. 2004. Nat. Cell Biol. 6, 849.
      Product Information
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7-8.
      Positive controlHeLa whole cell lysate or HeLa cell S100 extract
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1 µg/ml)
      Immunoprecipitation (2 µg/mg lysate protein)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids 4775-4829 of human BIRC6/Apollon
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1031 0

      Documentation

      Anti-Apollon (4775-4829) Rabbit pAb Certificates of Analysis

      TitleLot Number
      AP1031

      References

      Reference overview
      Qiu, X.B., et al. 2005. J. Biol. Chem. 280, 174.
      Ren, J., et al. 2005. Proc. Natl. Acad. Sci. USA 102, 565.
      Hao, Y., et al. 2004. Nat. Cell Biol. 6, 849.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-August-2007 RFH
      SynonymsAnti-BIRC6, Anti-Baculoviral IAP repeat-containing 6, Anti-BRUCE, Anti-KIAA1289
      ApplicationImmunoblotting (1 µg/ml)
      Immunoprecipitation (2 µg/mg lysate protein)
      Application Data
      Detection of human Apollon by immunoprecipitation and immunoblotting. Samples: Whole cell lysate (20 μg, lane 1, 50 μg, lane 2) from HeLa cells or S100 extract (20 μg, lane 3, 50 μg, lane 4) from HeLa cells. Antibody for IP: Anti-Apollon Rabbit pAb (Cat. No. AP1031) (2 μg/mg lysate protein). Antibody for IB: Anti-Apollon Rabbit pAb (Cat. No. AP1031) (1 μg/ml). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~500 kDa Apollon protein.
      BackgroundApollon (Q9NR09) is a ~500 kDa membrane associated inhibitor of apoptosis (IAP) protein. It encodes baculoviral-IAP-repeat (BIR) and ubiquitin-conjugating enzyme (UBC) domains at the N and C-termini, respectively. Unlike other IAPs Apollon encodes a chimeric E2/E3 ubiquitin ligase and is essential for inhibition of apoptosis in certain cell lines. Apollon inhibits apoptosis by promoting ubiquitination and degradation of active Smac and caspase-9, but not caspase-3. Unlike other IAPs, Apollon also binds to and inactivates precursors of caspase-9 and Smac. Inactivation of Apollon also leads to upregulation and nuclear localization of p53, which results in activation of caspase-2, Bax, and Bak, ultimately leading to mitochondrial apoptosis.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids 4775-4829 of human BIRC6/Apollon
      IsotypeIgG
      Specieshuman
      Positive controlHeLa whole cell lysate or HeLa cell S100 extract
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7-8.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage.
      Toxicity Standard Handling
      ReferencesQiu, X.B., et al. 2005. J. Biol. Chem. 280, 174.
      Ren, J., et al. 2005. Proc. Natl. Acad. Sci. USA 102, 565.
      Hao, Y., et al. 2004. Nat. Cell Biol. 6, 849.