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PC529 Anti-BACE (Ab-2) (485-501) Rabbit pAb

Anti-BACE (Ab-2) (485-501) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-β-Amyloid Precursor Protein Cleaving Enzyme, Anti-ASP2, Anti-β-Secretase

PC529
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~70-75 kDa BACE protein in human brain tissue extracts and mouse NIH3T3 cell lysates.
      Catalogue NumberPC529
      Brand Family Calbiochem®
      SynonymsAnti-β-Amyloid Precursor Protein Cleaving Enzyme, Anti-ASP2, Anti-β-Secretase
      Application Data
      Detection of human BACE by immunoblotting. Sample: Whole tissue extract from human brain (lanes A,B) and whole cell lysate from NIH3T3 cells (lane C). Primary antibody: Anti-BACE (Ab-2) (485-501) Rabbit pAb (Cat. No. PC529) (1 µg/ml) pre-incubated without blocking peptide (lanes A,C) or with blocking peptide (lane B). Detection: chemiluminescence.
      References
      ReferencesHussain, I., et al. 1999. Mol. Cell. Neurosci. 14, 419.
      Sinha, S., et al. 1999. Nature. 502, 537.
      Vassar, R., et al. 1999. Science. 266, 735.
      Yan, R., et al. 1999. Nature 402, 583.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlHuman brain tissue lysate
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:500-1:1000)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (CLRQQHDDFADDISLLK) corresponding to amino acids 485-501 of human BACE
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC529 0

      Documentation

      Anti-BACE (Ab-2) (485-501) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-BACE (Ab-2) (485-501) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC529

      References

      Reference overview
      Hussain, I., et al. 1999. Mol. Cell. Neurosci. 14, 419.
      Sinha, S., et al. 1999. Nature. 502, 537.
      Vassar, R., et al. 1999. Science. 266, 735.
      Yan, R., et al. 1999. Nature 402, 583.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-October-2007 RFH
      SynonymsAnti-β-Amyloid Precursor Protein Cleaving Enzyme, Anti-ASP2, Anti-β-Secretase
      ApplicationImmunoblotting (1:500-1:1000)
      Application Data
      Detection of human BACE by immunoblotting. Sample: Whole tissue extract from human brain (lanes A,B) and whole cell lysate from NIH3T3 cells (lane C). Primary antibody: Anti-BACE (Ab-2) (485-501) Rabbit pAb (Cat. No. PC529) (1 µg/ml) pre-incubated without blocking peptide (lanes A,C) or with blocking peptide (lane B). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody purified by ion exchange chromotography. Recognizes the ~70-75 kDa BACE protein.
      BackgroundAccumulation of the amyloid-β(Aβ) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Aβ peptide is generated by proteolytic cleavage of the β-amyloid protein precursor (APP) at β- and γ-sites by two proteases. APP is first cleaved by β-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as a substrate for γ-secretase to generate the ~4 kDa amyloid-βpeptide, which is deposited in the brains of all suffers of Alzheimers disease. The long-sought β-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). BACE, a ~70 kDa protein, is a novel transmembrane aspartic protease that colocalizes with APP.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (CLRQQHDDFADDISLLK) corresponding to amino acids 485-501 of human BACE
      IsotypeIgG
      Specieshuman, mouse
      Positive controlHuman brain tissue lysate
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesHussain, I., et al. 1999. Mol. Cell. Neurosci. 14, 419.
      Sinha, S., et al. 1999. Nature. 502, 537.
      Vassar, R., et al. 1999. Science. 266, 735.
      Yan, R., et al. 1999. Nature 402, 583.