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MABF3243-25UG Anti-CD1d Antibody, clone CD1d42

Anti-CD1d, clone CD1d42 , Cat. No. MABF3243, is a mouse monoclonal antibody that detects CD1d and is tested for use in Flow Cytometry and Inhibition Assay.

MABF3243-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3243-25UG
      DescriptionAnti-CD1d Antibody, clone CD1d42
      Alternate Names
      • Antigen-presenting glycoprotein CD1d
      • R3G1
      Background InformationAntigen-presenting glycoprotein CD1d (UniProt: P15813; also known as R3G1, CD1d) is encoded by the CD1D gene (Gene ID: 912) in human. Cd1d is a single-pass type 1 membrane glycoprotein, which is synthesized with a signal peptide (aa 1-19) that is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 20-301), a transmembrane domain (aa 302-322), and a short cytoplasmic domain (aa 323-335). It is expressed at high levels by human cortical thymocytes, and its expression is also observed on certain T cell leukemias. It serves as a key antigen-presenting molecule involved in the selection and activation of NK cells. It is a major-histocompatibility-complex (MHC) class-I-like molecule that presents self-lipid and non-self-lipid antigens to invariant natural killer T (iNKT) cells. Following engagement of the invariant T cell receptor (iTCR) by CD1d lipid complexes, iNKT cells proliferate, produce cytokines, and become cytotoxic, regulating innate and adaptive immune responses. (Ref.: Oleinika, K., et al. (2018). Nat. Commun. 9(1); Article 684; Spada, FM., et al. (2000). Eur. J. Immunol. 30; 3468-3477; Exley, M., et al. (2000). Immunology. 100(1): 37-47).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-CD1d, clone CD1d42 , Cat. No. MABF3243, is a mouse monoclonal antibody that detects CD1d and is tested for use in Flow Cytometry and Inhibition Assay.
      Key Applications
      • Flow Cytometry
      • Inhibition
      Application NotesTested applications

      Inhibition Assay: A representative lot inhibited the responses of NK T cell clones to -GalCer in the presence of monocyte lineage APC. (Spada, F.M., et al. (2000). Eur J Immunol. 30(12):3468-77).

      Flow Cytometry Analysis: A representative lot detected CD1d in Flow Cytometry (Spada, F.M., et al. (2000). Eur J Immunol. 30(12):3468-77).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenRecombinant fusion protein composed of 1, 2 and 3 domains of human CD1d fused to the Fc portion of murine IgG2a.
      EpitopeExtracellular domain
      CloneCD1d42
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone CD1d42 is a mouse monoclonal antibody that detects CD1d. It targets an epitope within the extracellular domain.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CD1D
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight37.72 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

      Flow Cytometry Analysis: 1.0 µg of this antibody detected CD1d in one million Human peripheral blood mononuclear cells (PBMC).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3243-25UG 04065269126089

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