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218794 Anti-Caspase-9 (1-134) Rabbit pAb

Anti-Caspase-9 (1-134) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-ICE-LAP6, Anti-Mch6, Anti-APAF-3, Anti-CASP9

218794
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~46 kDa latent form of caspase-9 in heat shocked HeLa cells and the ~37 kDa, ~18 kDa (weakly), and ~10 kDa processed forms of casapase-9.
Catalogue Number218794
Brand Family Calbiochem®
SynonymsAnti-ICE-LAP6, Anti-Mch6, Anti-APAF-3, Anti-CASP9
Application Data
Detection of human caspase-9 by immunoblotting. Samples: Whole cell lysates from Jurkat cells left untreated (lane 1) and apoptosis-inducing agents (lane 2). Primary antibody: Anti-Caspase-9 (1-134) Rabbit pAb (Cat. No. 218794) (2 µg/ml). Detection: chemiluminescence.
References
ReferencesFearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
Garrido, C., et al. 1999. FASEB J. 13, 2061.
Krajewski, S., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 5752.
Duan, H., et al. 1996. J. Biol. Chem. 271, 16720.
Srinivasula, S.M., et al. 1996. J. Biol. Chem. 271, 27099.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlHeat-shocked HeLa cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:500)
Application CommentsThis antibody recognizes both active caspase-9 enzyme and pro-caspase-9. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena recombinant protein containing amino acids 1-134 of human caspase-9 fused to a His•Tag® sequence
ImmunogenHuman
HostRabbit
IsotypeIgG
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
218794 0

Documentation

Anti-Caspase-9 (1-134) Rabbit pAb Certificates of Analysis

TitleLot Number
218794

References

Reference overview
Fearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
Garrido, C., et al. 1999. FASEB J. 13, 2061.
Krajewski, S., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 5752.
Duan, H., et al. 1996. J. Biol. Chem. 271, 16720.
Srinivasula, S.M., et al. 1996. J. Biol. Chem. 271, 27099.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-July-2007 RFH
SynonymsAnti-ICE-LAP6, Anti-Mch6, Anti-APAF-3, Anti-CASP9
ApplicationImmunoblotting (1:500)
Application Data
Detection of human caspase-9 by immunoblotting. Samples: Whole cell lysates from Jurkat cells left untreated (lane 1) and apoptosis-inducing agents (lane 2). Primary antibody: Anti-Caspase-9 (1-134) Rabbit pAb (Cat. No. 218794) (2 µg/ml). Detection: chemiluminescence.
DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~46 kDa, latent, the ~34-37 kDa active large subunit, and an ~18 kDa (weakly) and ~10 kDa (weakly) intermediates of caspase-9.
BackgroundCaspase-9 is activated by the proteolysis of pro-caspase-9 by CPP32 (caspase-3) and granzyme B (caspase-8). It is composed of a 34-37 kDa large subunit and a 10 kDa small subunit.
HostRabbit
Immunogen speciesHuman
Immunogena recombinant protein containing amino acids 1-134 of human caspase-9 fused to a His•Tag® sequence
IsotypeIgG
Speciesbovine, chicken, guinea pig, human, monkey, mouse, rat, sheep
Positive controlHeat-shocked HeLa cells
FormLiquid
FormulationIn PBS.
Preservative≤0.1% sodium azide
CommentsThis antibody recognizes both active caspase-9 enzyme and pro-caspase-9. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesFearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
Garrido, C., et al. 1999. FASEB J. 13, 2061.
Krajewski, S., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 5752.
Duan, H., et al. 1996. J. Biol. Chem. 271, 16720.
Srinivasula, S.M., et al. 1996. J. Biol. Chem. 271, 27099.