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MABF3161-25UG Anti-β-Catenin Antibody, clone 12F7

Anti-β-Catenin, clone 12F7, Cat. No. MABF3161, is a mouse monoclonal antibody that detects -β-Catenin and is tested for use in Immunohistochemistry and Western Blotting.

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MABF3161-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3161-25UG
      DescriptionAnti-β-Catenin Antibody, clone 12F7
      Alternate Names
      • b-Cat
      Background Informationβ-Catenin (UniProt: O42486; also known as b-Cat) is encoded by the BCat gene (Gene ID: 395964) in chicken. Wnt/β-catenin signaling, a highly conserved pathway through evolution, regulates key cellular functions including proliferation, differentiation, migration, genetic stability, apoptosis, and stem cell renewal. β-catenin is a key downstream component of the canonical Wnt signaling pathway that participates in intercellular adhesion and Wnt-mediated transcriptional activation. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1, and GSK-3β that promotes phosphorylation on N-terminal serine and threonine residues that leads to its subsequent degradation by the proteasome. In the presence of Wnt ligand, it is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, which leads to activation of Wnt responsive genes. O-glycosylation at serine 23 is reported to decreases its nuclear localization and transcriptional activity. Wnt/β-catenin signaling is shown to be essential for embryonic stem cell (ESC) pluripotency and differentiation. In ESC β-catenin is trimethylated on Lysine 49 by Ezh2 methyltransferase and this trimethylated form represses neuronal differentiation genes Sox1 and Sox3. Mutations in β-catenin gene in humans are reported to cause colorectal cancers, medulloblastoma, and ovarian cancers. (Ref.: Hoffmeyer, K., et al. (2017). Cell Reports 18(12); 2815-2824; Pai, SC., et al. (2017). J. Hematol. Oncol. 10; Article 101; Johnson, KR., et al. (1993). Exp. Cell Res. 207(2); 252-260).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-β-Catenin, clone 12F7, Cat. No. MABF3161, is a mouse monoclonal antibody that detects -β-Catenin and is tested for use in Immunohistochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesTested Applications

      Immunohistochemistry Applications: A representative lot detected β-Catenin in Immunohistochemistry applications (Qu, Y., et al. (2016). Proc. Natl. Acad. Sci. USA.113(33); 9339-44).

      Western Blotting Analysis: A representative lot detected β-Catenin in Western Blotting applications (Johnson, K.R., et al. (1993). Exp. Cell Res. 207(2); 252-60).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenMBP-conjugated recombinant full-length chicken β-catenin.
      EpitopeN-terminal half
      Clone12F7
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 12F7 is a mouse monoclonal antibody that detects β-catenin. It targets an epitope within 22 amino acids from the N-terminal half.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Chicken, Bovine, Moue, Rat, Xenopus, Zebrafish based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • B-Cat
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~95 kDa observed; 85.44 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysates.

      Western Blotting Analysis: A 1:125 dilution of this antibody detected β-Catenin in A431 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3161-25UG 04065268529133