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MABN2276-25UL Anti-Dab1 Antibody, clone 4E12

Anti-Dab1, clone 4E12, Cat. No. MABN2276, is a highly specific rat monoclonal antibody that targets Disabled homolog 1 and has been tested in Immunohistochemistry, Immunoprecipitation, and Western Blotting.

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MABN2276-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2276-25UL
      DescriptionAnti-Dab1 Antibody, clone 4E12
      Alternate Names
      • Disabled homolog 1
      Background InformationDisabled homolog 1 (UniProt: P97318; also known as DAB1) is encoded by the Dab1 gene (Gene ID: 13131) in murine species. Dab 1 is mainly expressed in the brain and serves as an adapter molecule involved in neural development where it is reported to direct the migration of cortical neurons past previously formed neurons to reach their proper layer. It is a cytosolic protein that is shown to be essential component of the reelin signaling pathway. It is shown to bind to the cytoplasmic tail of Reelin receptor, APO E receptor 2, and to LDL receptor. Dab1 can be phosphorylated on Tyr198, Tyr220, and Tyr232 upon reelin induction in embryonic neurons. It can also be phosphorylated on Ser524, but this phosphorylation is independent of reelin signaling. Tyrosine phosphorylation of Dab1 by Src family tyrosine kinases is considered to be the most critical downstream event in Reelin signaling. Dab1 contains a phosphotyrosine interaction domain (PID) (aa 36-189), which specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins. Seven different isoforms of Dab1 have been identified that are generated via alternative splicing. Clone 4E12 binds to an epitope within amino acids 161-320. (Ref.: Onoue, A., et al. (2014). Neurosci. Res. 88; 23-27).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Dab1, clone 4E12, Cat. No. MABN2276, is a highly specific rat monoclonal antibody that targets Disabled homolog 1 and has been tested in Immunohistochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Dab1 in Western Blotting applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27; Nakamura, K., et. al. (2016). Neuroscience. 336:20-29).

      Immunoprecipitation Analysis: A 1:500 dilution from a representative lot immunoprecipitated Dab1 in cerebral cortex tissue lysate from P20 mouse (Courtesy of Prof. Mitsuharu Hattori, Nagoya City University).

      Immunohistochemistry Analysis: A representative lot detected Dab1 in Immunohistochemistry applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27).
      Biological Information
      ImmunogenGST-tagged full-length recombinant murine Disabled homolog 1 expressed in E. coli.
      Clone4E12
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 4E12 is a rat monoclonal antibody that detects murine Disabled homolog 1 (Dab1).
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Dab1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~78 and 62 kDa observed; 63.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293T cells expressing murine Dab 1.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Dab1 in lysates from HEK293T cells expressing murine Dab 1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2276-25UL 04054839638596

      Documentation