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CP44 Anti-Endothelin1 (Ab-1) Mouse mAb (TR.ET.48.5)

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CP44
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes ET-1 in endothelial cells and vascular tissue. Little to no cross-reactivity with related peptides.
      Catalogue NumberCP44
      Brand Family Calbiochem®
      Application Data
      Detection of human endothelin1 by immunohistochemistry. Sample: Human bowel tissue. Primary antibody: Anti-Endothelin1 (Ab-1) Mouse mAb (TR.ET.48.5) (Cat. No. CP44) (1:250). Detection: fluorescence.

      Detection of human endothelin1 by immunohistochemistry. Sample: Human bowel tissue. Primary antibody: Anti-Endothelin1 (Ab-1) Mouse mAb (TR.ET.48.5) (Cat. No. CP44) (1:250). Detection: fluorescence.
      References
      ReferencesTraish, A., et al. 1992. Hybridoma 11, 147.
      Product Information
      FormLiquid
      FormulationAscites diluted in PBS.
      Positive controlEndothelial cells or vascular tissue
      Preservative≤0.1% sodium azide
      Applications
      Application ReferencesOriginal Clone Traish, A., et al. 1992. Hybridoma 11, 147.
      Key Applications Immunocytochemistry
      Paraffin Sections
      Radioimmunoassay
      Application NotesImmunocytochemistry (1:250)
      Paraffin Sections (1:250, trypsin pre-treatment required)
      Radioimmunoassay (1:25,000)
      Application CommentsClone TR.ET.48.5 shows 100% cross-reactivity with ET-1, less than 10% with ET-2 and ET-3, 0% with sarafotoxin and C-terminal hexapeptide of ET-1. It shows no cross-reactivity with β-endorphin, neuropeptide YY, somatostatin, secretin, or calcitonin gene-related peptide. This antibody is useful for localizing and determining the distribution of ET-1 by immunocytochemistry in 10% formalin fixed cell types. For paraffin sections, pre-treat with trypsin/PBS for 10 min at room temperature. Pure ET-1 will neutralize this antibody and block ET-1 staining. Concentration of ET-1 in solution (serum/plasma, milk, urine) can be determined by competitive RIA. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified ET-1
      CloneTR.ET.48.5
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C)
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CP44 0

      Documentation

      Anti-Endothelin1 (Ab-1) Mouse mAb (TR.ET.48.5) Certificates of Analysis

      TitleLot Number
      CP44

      References

      Reference overview
      Traish, A., et al. 1992. Hybridoma 11, 147.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision31-January-2008 JSW
      ApplicationImmunocytochemistry (1:250)
      Paraffin Sections (1:250, trypsin pre-treatment required)
      Radioimmunoassay (1:25,000)
      Application Data
      Detection of human endothelin1 by immunohistochemistry. Sample: Human bowel tissue. Primary antibody: Anti-Endothelin1 (Ab-1) Mouse mAb (TR.ET.48.5) (Cat. No. CP44) (1:250). Detection: fluorescence.

      Detection of human endothelin1 by immunohistochemistry. Sample: Human bowel tissue. Primary antibody: Anti-Endothelin1 (Ab-1) Mouse mAb (TR.ET.48.5) (Cat. No. CP44) (1:250). Detection: fluorescence.
      DescriptionMouse monoclonal antibody generated by immunizing BALB/c x A/JF1 with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells (see application references). Supplied as diluted ascites. Recognizes ET-1.
      BackgroundEndothelin is an extremely potent vasoconstrictor in vascular and non-vascular smooth muscle that is also involved in the regulation of cell proliferation and maintenance of the extracellular matrix. Three distinct 21 amino acid endothelin isoforms have been identified (ET-1, ET-2, ET-3) which are processed from endothelin precursor proteins by an endothelin converting enzyme. ET-1, ET-2 and ET-3 are produced in endothelial and epithelial cells and can mediate biological effects in cells and tissues. These three isoforms have been shown to bind an ET receptor in lung, kidney, heart and liver.
      HostMouse
      Immunogenpurified ET-1
      CloneTR.ET.48.5
      IsotypeIgG₁
      Specieshuman, mouse, ovine, rat
      Positive controlEndothelial cells or vascular tissue
      FormLiquid
      FormulationAscites diluted in PBS.
      Preservative≤0.1% sodium azide
      CommentsClone TR.ET.48.5 shows 100% cross-reactivity with ET-1, less than 10% with ET-2 and ET-3, 0% with sarafotoxin and C-terminal hexapeptide of ET-1. It shows no cross-reactivity with β-endorphin, neuropeptide YY, somatostatin, secretin, or calcitonin gene-related peptide. This antibody is useful for localizing and determining the distribution of ET-1 by immunocytochemistry in 10% formalin fixed cell types. For paraffin sections, pre-treat with trypsin/PBS for 10 min at room temperature. Pure ET-1 will neutralize this antibody and block ET-1 staining. Concentration of ET-1 in solution (serum/plasma, milk, urine) can be determined by competitive RIA. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C)
      Toxicity Standard Handling
      ReferencesTraish, A., et al. 1992. Hybridoma 11, 147.
      Application referencesOriginal Clone Traish, A., et al. 1992. Hybridoma 11, 147.