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ABE2922-25UG Anti-Histone H1E frameshift mutant

Anti-Histone H1E frameshift mutant, Cat. No. ABE2922, is a rabbit polyclonal antibody that detects Histone H1E frameshift mutant and is tested for use in Affinity Binding Assay, Immunofluorescence, and Western Blotting.

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ABE2922-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABE2922-25UG
      DescriptionAnti-Histone H1E frameshift mutant
      Background InformationHistone 1, H1e (UniProt: A3R0T8; also known as Histone H1.4, HIST1H1E) is encoded by the HIST1H1E (also known as hCG_16457421) gene (Gene ID: 3008) in human. Histones have been classified under five major classes. The core histones H2A, H2B, H3, and H4 constitute the protein components of nucleosome core particles and histone H1 is a linker histone that plays a role in chromatin folding. Depletion of histone H1 can cause dramatic changes in chromatin structure, global nucleosome spacing, and reduced chromatin compaction. Pathogenic mutations in HIST1H1E gene result in the formation of histone H1 that is less effective in neutralizing negatively charged linker DNA and display reduced DNA binding and protein-protein interactions due to truncation in key residues. These mutations are reported to result in a shift in reading frame in the C-terminal region and produce truncated proteins. Frameshift mutations in HIST1H1E have been linked to Rahman syndrome that is manifested in overgrowth with intellectual disabilities (OGID) in early infancy. Subjects with Rahman syndrome display a global tendency in reduction of methylation state. (Ref.: Andrea Ciolfi, et al. (2020). Clinical Epigenetics 12; Article 7; Tatton-Brown , K., et al. (2017). Am. J. Hum. Genet. 100(5); 725-736).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Histone H1E frameshift mutant, Cat. No. ABE2922, is a rabbit polyclonal antibody that detects Histone H1E frameshift mutant and is tested for use in Affinity Binding Assay, Immunofluorescence, and Western Blotting.
      Key Applications
      • Western Blotting
      • Affinity Binding Assay
      • Immunofluorescence
      Application NotesTested Applications

      Immunofluorescence Analysis: A representative lot detected Histone H1E frameshift mutant in mESc and H1Eframeshift Knock-in cells (Courtesy of Prof. C. David Allis Lab at Rockefeller University).

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Histone H1E frameshift mutant in Human WBC and mESC's & various other cell lines (Courtesy of Prof. C. David Allis Lab at Rockefeller University).

      Affinity Binding Assay: A representative lot of this antibody bound Histone H1E peptide with a KD of 1.0 x 10-12

      in an affinity binding assay.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated Linear Peptide corresponding to the Unknown of Human Histone H1.
      EpitopeUnknown
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects Histone H1 with H1E frameshift mutation.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~33 kDa observed; 21.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human oligodendroglioma cell line SCC163 stably expressing transgenic H1Efs-FLAG-HA.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Histone H1E frameshift mutant in human oligodendroglioma cell line SCC163 stably expressing transgenic H1Efs-FLAG-HA.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE2922-25UG 04065267327860