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ABS2090-25UL Anti-ICDH

Anti-ICDH, Cat. No. ABS2090, is a rabbit polyclonal antibody that detects Isocitrate dehydrogenase [NADP]. It has been tested for use in Western Blotting.

Research Category: Signaling Gene Symbol: ICDH UniProt Number: No verified Uniprot, PUBMED 25977442
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ABS2090-25UL
25 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      E. coliWBRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABS2090-25UL
      DescriptionAnti-ICDH
      Alternate Names
      • Isocitrate dehydrogenase
      • ICDH (NADP)
      • IDH
      Background InformationIsocitrate dehydrogenase [NADP] (also known as IDH, ICDH) catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate. In eukaryotes there are at least three isozymes of IDH: two are located in the mitochondrial matrix (one NAD+-dependent, the other NADP+-dependent), while the third one (also NADP+-dependent) is cytoplasmic. In E.coli the activity of a NADP+-dependent ICDH is controlled by the phosphorylation of a serine residue and the phosphorylated form of ICDH is completely inactivated. It has been reported that if Ser113 is replaced by alanine, cysteine, threonine, or tyrosine there is some loss of enzymatic activity. However, substitution by aspartic acid completely abolishes its activity. ICDH contains three domains: a large alpha + beta domain, a small alpha/beta domain and an alpha/beta clasp-like domain involving both subunits. (Ref.: Hurley, JH et al (1989). Proc. Natl. Acad. Sci. USA. 86 (22); 8635-8639).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
      Applications
      ApplicationAnti-ICDH, Cat. No. ABS2090, is a rabbit polyclonal antibody that detects Isocitrate dehydrogenase [NADP]. It has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected ICDH in 20 UL Legionella cell lysate.
      Western Blotting Analysis: A representative lot detected ICDH in E. Coli cell lysate (Xu, L., et. al. (2010). PLoS Pathog. 6(3):e1000822; Lu, C., et. al. (2016). Sci Signal. 9(412):ra11).
      Biological Information
      ImmunogenHis-tagged full length recombinant isocitrate dehydrogenase from Legionella pneumophila, strain Lp02.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects isocitrate dehydrogenase in E. coli and Legionella pneumophila.
      IsotypeIgG
      Species Reactivity
      • E. coli
      Species Reactivity NoteE. Coli, Legionella pneumophila.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • ICDH
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight45 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in E.Coli cell lysate.
      Western Blotting Analysis: A 1:10,000 dilution of this antibody detected ICDH in 20 UL E.Coli cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS2090-25UL 04054839505577

      Documentation