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MABF3174-25UG Anti-IL-17A Antibody, clone 6F9.2.12.1.5

Anti-IL-17A, clone 6F9.2.12.1.5, Cat. No. MABF3174, is a mouse monoclonal antibody that detects IL-17A and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

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MABF3174-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3174-25UG
      DescriptionAnti-IL-17A Antibody, clone 6F9.2.12.1.5
      Alternate Names
      • Interleukin-17A
      • IL-17
      • Cytotoxic T-lymphocyte-associated antigen 8
      • CTLA-8
      Background InformationInterleukin-17A (UniProt: Q16552; also known as IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8) is encoded by the IL17A (also known as CTLA8, IL17) gene (Gene ID: 3605) in human. IL-17A is a proinflammatory cytokine secreted by activated CD4+ Th17 cells. It is synthesized with a signal peptide (aa 1-23) that is subsequently cleaved off to produce the mature protein. Its levels are up-regulated by IL23A-IL12B. IL-17 is reported to play an important role in multiple biological processes, including the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kB and co-stimulation of T cell proliferation. It is also involved in antimicrobial host defense and maintenance of tissue integrity. Its actions are mediated via interaction with IL-17RA and IL-17RC heterodimeric receptor complex, triggering homotypic interaction of these receptors with (TRAF3 Interacting Protein 2 (TRAF3IP2) adapter. This leads to downstream TRAF6-mediated activation of NF-kB and MAP kinase pathways resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides, and matrix metalloproteinases. IL-17A also plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. In synergy with IL-17F, it is shown to mediate the production of antimicrobial b-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, which limits the entry of microbes through the epithelial barriers. (Ref.: Boisson, B., et al. (2013). Immunity. 39(4); 676-686; Wright, J.F., et al. (2008). J. Immunol. 181(4); 2799-2805).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-IL-17A, clone 6F9.2.12.1.5, Cat. No. MABF3174, is a mouse monoclonal antibody that detects IL-17A and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesTested Applications

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-17A in human tonsil tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenA recombinant fragment corresponding to amino acids 19 to 155 of human IL-17A.
      EpitopeUnknown
      Clone6F9.2.12.1.5
      Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 6F9.2.12.1.5 is a mouse monoclonal antibody that specifically detects human Interleukin-17A.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IL17A
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~30 kDa observed; 17.5 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant human IL-17A.

      Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant human IL-17A.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3174-25UG 04065267327846