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MABT821 Anti-Integrin beta1 (CD29) Antibody, clone mAb13

Anti-Integrin beta1 (CD29), clone mAb13, Cat. No. MABT821, is a rat monoclonal antibody that detects integrin beta-1 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Inhibition studies.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: ITGB1, FNRB, MDF2, MSK12 UniProt Number: P05556
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MABT821
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIHC, IP, InhibitionRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT821
      DescriptionAnti-Integrin beta1 (CD29) Antibody, clone mAb13
      Alternate Names
      • Fibronectin receptor subunit beta
      • Glycoprotein IIa
      • GPIIA
      • VLA-4 subunit beta
      • CD29
      Background InformationIntegrin beta-1 (UniProt: P05556; also known as Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12) gene (Gene ID: 3688) in human. Integrin beta-1 is a single-pass type I membrane protein that can associate with one of the multiple alpha subunits to serve as a receptor for fibronectin and other extracellular matrix proteins. Integrin beta-1 integrin recognizes the sequence R-G-D in a wide array of ligands. Five isoforms of integrin beta-1 have been reported that are produced by alternative splicing. Integrin beta-1 is synthesized with a signal peptide (aa 1-20) that is subsequently cleaved off. The mature form has an extracellular domain (aa 21-728), a helical domain (aa 729-751) and a cytoplasmic region (aa 752-798). Isoform 1 of integrin beta-1 is widely expressed and other isoforms are generally co-expressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4 is selectively expressed in peripheral T-cells and isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle). Isoform 2 is reported to interfere with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in PBS without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Integrin beta1 (CD29), clone mAb13, Cat. No. MABT821, is a rat monoclonal antibody that detects integrin beta-1 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Inhibition studies.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Inhibition
      Application NotesInhibition Analysis: A representative lot blocked cell attachment in Inhibition studies (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75; Humphries, J.D., et. al. (2005). J Biol Chem. 280(11):10234-43).

      Immunocytochemistry Analysis: A representative lot detected Integrin beta1 (CD29) in Immunocytochemistry applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75; Humphries, J.D., et. al. (2005). J Biol Chem. 280(11):10234-43).

      Immunoprecipitation Analysis: A representative lot detected Integrin beta1 (CD29) in Immunoprecipitation applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75).

      Inhibition Analysis: A representative lot blocked HT-1080 cells adhesion to fibronectin coated plates (Courtesy of Dr. Kenneth M. Yamada, M.D., Ph.D., NIDCR, National Institutes of Health, Bethesda, MD USA).
      Biological Information
      ImmunogenFibronectin receptor (alpha V beta 1) purified from human placenta.
      ClonemAb13
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone mAb13 specifically detects beta 1 subunit of human integrin. It targets an epitope within 12 amino acids from the N-terminal half.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ITGB1
      • FNRB
      • MDF2
      • MSK12
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight88.42 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Integrin beta1 (CD29) in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT821 04054839396762

      Documentation

      Anti-Integrin beta1 (CD29) Antibody, clone mAb13 Certificates of Analysis

      TitleLot Number
      Anti-Integrin beta1 (CD29), clone mAb13 - 3172422 3172422
      Anti-Integrin beta1 (CD29), clone mAb13 - 3216471 3216471
      Anti-Integrin beta1 (CD29), clone mAb13 - 3291390 3291390
      Anti-Integrin beta1 (CD29), clone mAb13 - 3449078 3449078
      Anti-Integrin beta1 (CD29), clone mAb13 - 3678854 3678854
      Anti-Integrin beta1 (CD29), clone mAb13 - 3726498 3726498
      Anti-Integrin beta1 (CD29), clone mAb13 - 3837763 3837763
      Anti-Integrin beta1 (CD29), clone mAb13 - 3889895 3889895
      Anti-Integrin beta1 (CD29), clone mAb13 - 3921448 3921448
      Anti-Integrin beta1 (CD29), clone mAb13 - 3959150 3959150
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