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MABF2781-25UL Anti-LCMV Nucleoprotein Antibody, clone VL-4

Anti-LCMV Nucleoprotein, clone VL-4, Cat. No. MABF2781, is a rat monoclonal antibody that detects LCMV nucleoprotein and is tested for use in Activity Assay, ELISA, Flow Cytometry, Immunohistochemistry, and Neutralizing.

MABF2781-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2781-25UL
      DescriptionAnti-LCMV Nucleoprotein Antibody, clone VL-4
      Alternate Names
      • LCMV Nucleocapsid Protein
      • Protein N
      Background InformationNucleoprotein (UniProt: P07400; also known as Nucleocapsid protein, Protein N) is encoded by the N gene (Gene ID: 127897) in Lymphocytic choriomeningitis virus (LCMV). LCMV is a murine enveloped RNA virus of the arenavirus family. Its viral surface is densely covered with viral fusion glycoprotein spikes that form heterodimeric trimers, consisting of the peripheral receptor binding glycoprotein 1 (GP-1) and a transmembrane subunit (GP-2). The large segment of LCMV genome encodes for the RNA-dependent-RNA-polymerase (L) and the matrix protein (Z) that mediates viral assembly and budding. Its small segment (S) encodes the glycoprotein precursor (GPC) and the viral nucleoprotein (NP). GPC is then co-translationally cleaved by signal peptidase to produce a stable 58 amino acid stable signal peptide (SSP) and GPC that is post-translationally processed by the cellular site 1 protease to form GP-1 and GP-2. GP-1 is reported to mediate virion attachment to the cell surface followed by cell entry via receptor-mediated endocytosis. The GP-2 subunit is responsible for the pH-dependent fusion event in the acidic environment of the endosome to complete the virus cell entry process and release of virus ribonucleoprotein into the cell cytoplasm to initiate transcription and replication of the viral genome. The nucleoprotein of LCMV encapsidates the genome and protects it from nucleases. The encapsidated genomic RNA known as the nucleocapsid (NC) serves as template for viral transcription and replication. Clone VL-4 specifically reacts with LCMV nucleoprotein and does not exhibit reactivity with vaccinia or influenza virus infected cells. (Ref.: Urata, S., et al. (2018). PLoS Pathog 14(7): e1007172; Eschli, B., et al. (2007). J. Virol. 81(21); 11650-11657).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-LCMV Nucleoprotein, clone VL-4, Cat. No. MABF2781, is a rat monoclonal antibody that detects LCMV nucleoprotein and is tested for use in Activity Assay, ELISA, Flow Cytometry, Immunohistochemistry, and Neutralizing.
      Key Applications
      • ELISA
      • Activity Assay
      • Flow Cytometry
      • Neutralizing
      • Immunohistochemistry
      Application NotesTested applications

      ELISA Analysis: A representative lot detected LCMV Nucleoprotein in ELISA application (Eschli, B., et al. (2007). J Virol. 81(21):11650-7).

      Flow Cytometry Analysis: A representative lot detected LCMV Nucleoprotein in Flow Cytometry application (Herz, J., et al. (2015). J Exp Med. 212(8):1153-69).

      Neutralizing: A representative lot detected LCMV Nucleoprotein in Neutralizing applications (Eschli, B., et al. (2007). J Virol. 81(21):11650-7).

      Activity Assay: A representative lot detected Nucleoprotein in lymphocytic choriomeningitis virus (LCMV) (Data courtesy of the Division of Experimental Virology, Department of Biomedicine, University of Basel, Switzerland).

      Immunohistochemistry Applications: A representative lot detected LCMV Nucleoprotein in Immunohistochemistry applications (Herz, J., et al. (2015). J Exp Med. 212(8):1153-69; Urata, S., et al. (2018). PLoS Pathog. 14(7):e1007172).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenLymphocytic choriomeningitis virus (LCMV).
      EpitopeUnknown
      CloneVL-4
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone VL-4 is a rat monoclonal antibody that detects nucleoprotein of Lymphocytic choriomeningitis virus (LCMV).
      IsotypeIgG2aκ
      Species Reactivity
      • Virus
      Species Reactivity NoteLymphocytic choriomeningitis virus (LCMV).
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • N
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight62.15 for LCMV Nucleoprotein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by ELISA with recombinant LCMV nucleoprotein .

      ELISA Analysis: A serial dilution of this antibody detected recombinant CLMV nucleoprotein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2781-25UL 04065269366867