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MABC1133-25UL Anti-Mucin13 Antibody, clone C14

Anti-Mucin13, clone C14, Cat. No. MABC1133, is a mouse monoclonal antibody that detects Mucin13 and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Analysis, and Western Blotting.

MABC1133-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1133-25UL
      DescriptionAnti-Mucin13 Antibody, clone C14
      Alternate Names
      • MUC-13
      Background InformationMucin-13 (UniProt: Q9H3R2; also known as MUC-13, Down-regulated in colon cancer 1) is encoded by the MUC13 (also known as DRCC1, RECC, UNQ6194/PRO20221) gene (Gene ID: 56667) in human. Transmembrane mucins are glycoproteins involved in barrier function in epithelial tissues. Mucin-13 is a single-pass type I membrane protein but may also exist in a soluble form. It is highly expressed in epithelial tissues, particularly those of the gastrointestinal and respiratory tracts. Mucin-13 is characterized by a tandem repeat (TR) domain that is rich in serine and threonine residues that act as glycosylation sites. It is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 19-421), a transmembrane domain (aa 422-442), and a cytoplasmic domain (aa 443-512). The signal peptide targets Mucin-13 for translation into the endoplasmic reticulum where initial oligosaccharides are added, and the protein is transported to the Golgi apparatus for further glycosylation and posttranslational modifications. Mucin-13 contains three EGF-like domains (aa 173-211; 322-361; and 363-404) and one highly conserved SEA domain (aa 212-336) that contains a cleavage site. Through its EGF-like domain, it can enhance ErbB2/HER2 and ErbB3 signaling by increasing the localization of ErbB2 and ErbB3 to the cell membrane and by suppressing ligand-induced receptor internalization. Aberrant expression of Mucin-13 has been reported in a variety of epithelial carcinomas, including gastric, colorectal, and ovarian cancers. (Ref.: Maher, DM., et al. (2011). Mol. Cancer Res. 9(5); 531-537).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG in PBS without azide.
      Applications
      ApplicationAnti-Mucin13, clone C14, Cat. No. MABC1133, is a mouse monoclonal antibody that detects Mucin13 and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Analysis, and Western Blotting.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunocytochemistry
      • Immunoprecipitation
      • Inhibits Activity/Function
      • Western Blotting
      Application NotesInhibition Analysis: A representative lot inhibited pancreatic tumor growth in a pancreatic tumor xenograft mouse model (Courtesy of Subhash Chauhan, Ph.D., University of Tennessee Health Science Center, USA).

      Western Blotting Analysis: A representative lot detected Mucin-13 in 293T MUC13 EGFP (transfected) versus 2093T EGFP (non-transfected) (Courtesy of Subhash Chauhan, Ph.D., University of Tennessee Health Science Center, USA).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated Mucin-13 in T84 and HPAFII membrane lysates (Courtesy of Subhash Chauhan, Ph.D., University of Tennessee Health Science Center, USA).

      Immunocytochemistry Analysis: A represntative lot detected Mucin-13 in Capan-1, Panc-1, OMC3, SKOV-3, T84, SW480, HPAFII, and MiaPaca cells (Courtesy of Subhash Chauhan, Ph.D., University of Tennessee Health Science Center, USA).

      Flow Cytometry Analysis: A represetative lot detected Mucin-13 in Capan-1, Panc-1, OMC3, SKOV-3, T84, SW480, HPAFII, and MiaPaca cells (Courtesy of Subhash Chauhan, Ph.D., University of Tennessee Health Science Center, USA).

      ELISA Analysis: A representative lot detected Mucin-13 in Sandwich ELISA for the detection of MUC13 (Courtesy of Subhash Chauhan, Ph.D., University of Tennessee Health Science Center, USA).
      Biological Information
      ImmunogenGST-tagged full-length human recombinant Mucin-13.
      CloneC14
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone C14 is a mouse monoclonal antibody that specifically detects human Mucin-13.
      IsotypeIgGκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MUC13
      • DRCC1
      • RECC
      • UNQ6194/PRO20221
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight54.60 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in T84 cells.

      Flow Cytometry Analysis: 1 µg of this antibody detected Mucin-13 in one million T84 colon adenocarcinoma cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1133-25UL 04061841099192