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OP180 Anti-Noxa Mouse mAb (114C307)

This Anti-Noxa Mouse mAb (114C307) is validated for use in Immunoblotting for the detection of Noxa.

Anti-Noxa Mouse mAb (114C307) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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OP180
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

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OP180-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~11 kDa Noxa protein in RL-7 cells.
      Catalogue NumberOP180
      Brand Family Calbiochem®
      Application Data
      Detection of human Noxa by immunoblotting. Sample: Whole cell lysate from RL-7 cells. Primary antibody: Anti-Noxa Mouse mAb (114C307) (Cat. No. OP180) (2 µg/ml). Detection: chemiluminescence.
      References
      ReferencesOda, E., et al. 2000. Science 288, 1053.
      Product Information
      FormLiquid
      FormulationIn PBS, 0.05% BSA.
      Negative controlNormal mouse serum
      Positive controlRL-7 cell lysate
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1-2 µg/ml, chemiluminescence)
      Application CommentsAntibody should be titrated for optimal results in individual system.
      Biological Information
      Immunogena recombinant protein consisting of human Noxa fused to GST
      ImmunogenHuman
      Clone114C307
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C;)
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OP180-100UG 04055977208849

      Documentation

      Anti-Noxa Mouse mAb (114C307) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Noxa Mouse mAb (114C307) Certificates of Analysis

      TitleLot Number
      OP180

      References

      Reference overview
      Oda, E., et al. 2000. Science 288, 1053.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure

      Citations

      Title
    • Yolanda Fernandez, et al. (2006) Chemical Blockage of the Proteasome Inhibitory Function of Bortezomib: Impact on Tumor Cell Death. Journal of Biological Chemistry 281, 1107-1118.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-October-2007 JSW
      ApplicationImmunoblotting (1-2 µg/ml, chemiluminescence)
      Application Data
      Detection of human Noxa by immunoblotting. Sample: Whole cell lysate from RL-7 cells. Primary antibody: Anti-Noxa Mouse mAb (114C307) (Cat. No. OP180) (2 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~11 kDa Noxa protein.
      BackgroundNoxa, a BH3 member of the Bcl-2 family, has recently been identified in human and mouse. Expression of the Noxa gene involves direct activation of its promoter by p53. Increased expression of Noxa occurs in normal thymocytes, but does not occur in p53-deficient thymocytes. Coimmunoprecipitation data suggests that Noxa may interact with other members of the Bcl-2 family, including Bcl-XL and Mcl-1. Data has also shown that blocking the endogenous Noxa induction results in the suppression of apoptosis. Treating cells with Noxa antisense oligonucleotides blocks radiation-induced apoptosis, and this suggests that Noxa may be a mediator of p53-dependent apoptosis.
      HostMouse
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of human Noxa fused to GST
      Clone114C307
      IsotypeIgG₁
      Specieshuman
      Positive controlRL-7 cell lysate
      Negative controlNormal mouse serum
      FormLiquid
      FormulationIn PBS, 0.05% BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual system.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C;)
      Toxicity Standard Handling
      ReferencesOda, E., et al. 2000. Science 288, 1053.
      Citation
    • Yolanda Fernandez, et al. (2006) Chemical Blockage of the Proteasome Inhibitory Function of Bortezomib: Impact on Tumor Cell Death. Journal of Biological Chemistry 281, 1107-1118.
      		•