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06-1048 Anti-PACT Antibody, domain 2

Anti-PACT Antibody, domain 2 detects level of PACT & has been published & validated for use in WB.

Anti-PACT Antibody, domain 2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunological Signaling Gene Symbol: RAX , PACT, HSD14 , DYT16 UniProt Number: O75569
06-1048
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBRbSerumPolyclonal Antibody
      Description
      Catalogue Number06-1048
      DescriptionAnti-PACT Antibody, domain 2
      Alternate Names
      • PKR-associated protein X
      • PKR-associating protein X
      • Protein activator of the interferon-induced protein kinase
      • Protein kinase
      • interferon-inducible double stranded RNA-dependent activator
      • protein kinase
      • interferon-inducible double stranded RNA dependent activator
      Background InformationPACT is also known as a protein activator of the interferon-induced protein kinase, PKR. PACT is a heterodimer with 3 double stranded RNA binding domains (DRBM) and acts as an antiviral mediator, growth regulator and signal transducer stimulated by stress. PACT activates EIF2AK2 in the absence of double stranded RNA binding via the DRBM domains. Suppression of PACT occurs in the presence of growth factor and is thought to be involved in dystonia type 16, which leads to axial muscle movement, Parkinsonian features and sardonic smile.
      References
      Product Information
      FormatSerum
      Control
      • Mouse brain lysate
      PresentationUnpurified rabbit polyclonal serum.
      Applications
      ApplicationAnti-PACT Antibody, domain 2 detects level of PACT & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic linear peptide corresponding to domain 2 of PACT.
      EpitopeDomain 2
      HostRabbit
      SpecificityThis antibody recognizes domain 2 of PACT.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteMouse. Predicted to cross-react with human (Rowe, T.M., et al. 2006) and rat based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. [provided by RefSeq].
      Gene Symbol
      • RAX
      • PACT
      • HSD14
      • DYT16
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Appears to have a pro-apoptotic function that may be suppressed in the presence of growth factor By similarity. Activates EIF2AK2 in absence of double stranded RNA (dsRNA).

      SUBUNIT: Heterodimer. Interacts with EIF2AK2 through its DRBM domains. Also able to interact with dsRNA. Ref.1

      PTM: Phosphorylated on serine By similarity.

      DISEASE: Defects in PRKRA are the cause of dystonia type 16 (DYT16) [MIM:612067]. Affected individuals have progressive, generalised, early-onset dystonia with axial muscle involvement, oromandibular (sardonic smile), laryngeal dystonia and, in some cases, parkinsonian features. Ref.14 Ref.15

      SIMILARITY: Contains 3 DRBM (double-stranded RNA-binding) domains.
      Molecular Weight~34 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on mouse brain lysate.

      Western Blot Analysis:
      A 1:1000 dilution of this antibody detected PACT on wild type mouse brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      06-1048 04053252282645

      Documentation

      Anti-PACT Antibody, domain 2 Certificates of Analysis

      TitleLot Number
      Anti-PACT, domain 2 - NG1584938 NG1584938

      References

      Reference overviewPub Med ID
      Binding of the influenza A virus NS1 protein to PKR mediates the inhibition of its activation by either PACT or double-stranded RNA.
      Li, Shoudong, et al.
      Virology, 349: 13-21 (2006)  2006

      		16466763 16466763
      A role of the double-stranded RNA-binding protein PACT in mouse ear development and hearing.
      Rowe, Theresa M, et al.
      Proc. Natl. Acad. Sci. U.S.A., 103: 5823-8 (2006)  2006

      		16571658 16571658
      The dsRNA binding protein family: critical roles, diverse cellular functions.
      Saunders, Laura R and Barber, Glen N
      FASEB J., 17: 961-83 (2003)  2003

      		12773480 12773480