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MABT841 Anti-Podoplanin Antibody, clone LpMab-12

Anti-Podoplanin, clone LpMab-12, Cat. No. MABT841, is a mouse monoclonal antibody that detects human and monkey podoplanin and has been tested for use in ELISA, Flow Cytometry, and Immunohistochemistry (Paraffin).

Anti-Podoplanin Antibody, clone LpMab-12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: PDPN, GP36, PSEC0003, PSEC0025 UniProt Number: Q86YL7
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MABT841
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Mk, HELISA, FC, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT841
      DescriptionAnti-Podoplanin Antibody, clone LpMab-12
      Alternate Names
      • Aggrus
      • Glycoprotein 36
      • Gp36
      • PA2.26 antigen
      • T1-alpha
      • T1A
      Background InformationPodoplanin (UniProt: Q86YL7; also known as Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A) is encoded by the PDPN (also known as GP36) gene (Gene ID: 10630) in human. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells, such as lymphatic endothelial cells and podocytes. It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. Six isoforms of podoplanin have been described that are produced by alternative splicing. It is synthesized with a signal peptide of 22 amino acids, which is cleaved to produce the mature form. Podoplanin contains an extracellular domain (aa 23-131), a helical domain (aa 132-152) and a cytoplasmic region (aa 153-162). Podoplanin possesses three tandem repeat of platelet aggregation-stimulating (PLAG) domains in its N-terminus. Among the PLAG domains, sialylated O-glycan on Thr52 of PLAG3 is essential for the binding to C-type lectin-like receptor-2 (CLEC-2). Interaction of human podoplanin with CLEC-2 mainly involves Glu47 and Asp48 in the platelet PLAG3. Clone LpMab-12 specifically detects sialylated O-Glycan on Thr52 of platelet aggregation-stimulating domain of human podoplanin. It also reacts with the synthetic glycopeptide of hPDPN, corresponding to 38 54 amino acids that carries 2-6 sialylated N-acetyl-D-galactosamine (GalNAc) on Thr52. The minimal epitope of LpMab-12 is identified as Asp49 Pro53 of hPDPN. (Ref.: Kato, Y et al. (2016). PLoS ONE 11(3): e0152912).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Podoplanin, clone LpMab-12, Cat. No. MABT841, is a mouse monoclonal antibody that detects human and monkey podoplanin and has been tested for use in ELISA, Flow Cytometry, and Immunohistochemistry (Paraffin).
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Podoplanin in human testis cancer tissue.

      ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).

      Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).

      Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).
      Biological Information
      ImmunogenLN229 glioma cells expressing human Podoplanin.
      CloneLpMab-12
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone LpMab-12 detects Podoplanin in human and monkey cells. It targets an epitope within the N-terminal half. It specifically detects Sialylated O-Glycan on Thr52 of platelet aggregation-stimulating domain of human podoplanin. The minimal epitope of LpMab-12 is identified as Asp49-Pro53.
      IsotypeIgG1κ
      Species Reactivity
      • Monkey
      • Human
      Species Reactivity NoteHuman, Monkey.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PDPN
      • GP36
      • PSEC0003
      • PSEC0025
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight16.70 kda calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human colon cancer tissue.

      Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Podoplanin in human colon cancer tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT841 04054839352621