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MABE1893-100UL Anti-RFC1 Antibody, clone 6

Anti-RFC1, clone 6, Cat. No. MABE1893, is a mouse monoclonal antibody that detects Replication factor C subunit 1 (RFC1) and is tested for use in Western Blotting.

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MABE1893-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1893-100UL
      DescriptionAnti-RFC1 Antibody, clone 6
      Alternate Names
      • Replication factor C subunit 1
      • Activator 1 140 kDa subunit
      • A1 140 kDa subunit
      • Activator 1 large subunit
      • Activator 1 subunit 1
      • DNA-binding protein PO-GA
      • Replication factor C 140 kDa subunit
      • RF-C 140 kDa subunit
      • RFC140
      • Replication factor C large subunit
      Background InformationReplication factor C subunit 1 (UniProt: P35251; also known as Activator 1 140 kDa subunit, A1 140 kDa subunit, Activator 1 large subunit, Activator 1 subunit 1, DNA-binding protein PO-GA, Replication factor C 140 kDa subunit, RF-C 140 kDa subunit, RFC140, Replication factor C large subunit) is encoded by the RFC1 (also known as RFC140) gene (Gene ID: 5981) in human. Replication factor C (RFC) is a multi-subunit DNA replication factor that is composed of five subunits of 140, 40, 38, 37, and 36 kDa size. These five subunits exist in all eukaryotes and are highly conserved in both structure and function. RFC1 is the largest subunit in this complex. RFC serves as a DNA-dependent ATPase that binds in a structure-specific manner to the 3 end of a primer hybridized to a template DNA. This ATPase is activated in the presence of primer/template DNA and is further stimulated by PCNA addition. In eukaryotes, it acts as a clamp loader that can bind and open the homotrimeric PCNA clamp, and then load PCNA onto a template-primer junction in an ATP-dependent reaction. Coupled with ATP hydrolysis, RFC is ejected from the PCNA clamp for the next round of DNA synthesis and then the clamp recruits DNA polymerases for processive elongation of DNA chains. In addition to its role in DNA replication, RFC also plays a role in DNA polymerase switching, DNA repair, and check-point control in cell cycle progression. RFC1 displays wide tissue distribution but is undetectable in placental tissue. (Ref.: Chen, Y., et al. (2018). Front. Plant Sci. 9; 779; Bunz, F., et a. (1993). Proc. Natl. Acad. Sci. USA. 90 (23); 11014-11018; Tsurimoto, T., and Stillman, B. (1989). EMBO J. 8(12); 3883-3889).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-RFC1, clone 6, Cat. No. MABE1893, is a mouse monoclonal antibody that detects Replication factor C subunit 1 (RFC1) and is tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected RFC1 in Western Blotting applications (Farina, A., et. al. (2008). J Biol Chem. 283(30):20925-36).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenPurified Replication factor C subunit 1 (RFC1) from HEK293 cell nuclear extract.
      EpitopeUnknown
      Clone6
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 6 is a mouse monoclonal antibody that detects Replication factor C subunit 1 (RFC1).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman, Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RFC1
      • RFC140
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~140 kDa observed; 128.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysates.

      Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Replication factor C subunit 1 (RFC1) in HepG2 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1893-100UL 04065266298208