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AP1054 Anti-Sphingosine Kinase 2 Rabbit pAb

Anti-Sphingosine Kinase 2 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-SPHK2

AP1054
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~75 kD sphingosine kinase 2 protein in transfected HEK293 cells and breast and hepatic carcinoma tissue.
      Catalogue NumberAP1054
      Brand Family Calbiochem®
      SynonymsAnti-SPHK2
      Application Data
      Detection of human sphingosine kinase 2 by immunoblotting. Samples: whole cell lysate from HEK293 cells transfected with SphK2 expressing a c-Myc tag. Primary antibodies: Anti-Sphingosine Kinase 2 Rabbit pAb (Cat. No. AP1054) (lane 1) and antibody specific for the c-Myc tag (lane 2). Detection: chemiluminescence.

      Detection of human sphingosine kinase 2 by staining paraffin sections. Samples: human breast carcinoma tissue. Primary antibody: Anti-Sphingosine Kinase 2 Rabbit pAb (Cat. No. AP1054). Detection: AEC.
      References
      ReferencesShu, X., et al. 2002.Mol. Cell. Biol. 22, 7758.
      Xia, P., et al. 2002.J. Biol. Chem. 277, 7996.
      Liu, H., et al. 2000.J. Biol. Chem. 275, 19513.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlBreast carcinoma
      Preservative≤ 0.1% sodium azide
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (1:1000)
      Immunoblotting (1:100-500)
      Paraffin Sections (1:50-100)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids near the C-terminus of human SPHK2, conjugated to KLH
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1054 0

      Documentation

      Anti-Sphingosine Kinase 2 Rabbit pAb Certificates of Analysis

      TitleLot Number
      AP1054

      References

      Reference overview
      Shu, X., et al. 2002.Mol. Cell. Biol. 22, 7758.
      Xia, P., et al. 2002.J. Biol. Chem. 277, 7996.
      Liu, H., et al. 2000.J. Biol. Chem. 275, 19513.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-December-2008 JSW
      SynonymsAnti-SPHK2
      ApplicationELISA (1:1000)
      Immunoblotting (1:100-500)
      Paraffin Sections (1:50-100)
      Application Data
      Detection of human sphingosine kinase 2 by immunoblotting. Samples: whole cell lysate from HEK293 cells transfected with SphK2 expressing a c-Myc tag. Primary antibodies: Anti-Sphingosine Kinase 2 Rabbit pAb (Cat. No. AP1054) (lane 1) and antibody specific for the c-Myc tag (lane 2). Detection: chemiluminescence.

      Detection of human sphingosine kinase 2 by staining paraffin sections. Samples: human breast carcinoma tissue. Primary antibody: Anti-Sphingosine Kinase 2 Rabbit pAb (Cat. No. AP1054). Detection: AEC.
      DescriptionProtein G purified rabbit polyclonal antibody. Recognizes the ~75 kD sphingosine kinase 2 protein.
      BackgroundSphingosine kinase (SphK) catalyzes the phosphorylation of the lipid, sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility, and differentiation. SphK is an important signaling enzyme that is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids near the C-terminus of human SPHK2, conjugated to KLH
      IsotypeIgG
      Specieshuman
      Positive controlBreast carcinoma
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤ 0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesShu, X., et al. 2002.Mol. Cell. Biol. 22, 7758.
      Xia, P., et al. 2002.J. Biol. Chem. 277, 7996.
      Liu, H., et al. 2000.J. Biol. Chem. 275, 19513.