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MABF3172-100UG Anti-Sup35p-NM Antibody, clone 4A5

Anti-Sup35p-NM, clone 4A5, Cat. No. MABF3172, is a rat monoclonal antibody that detects of Sup35/HEFE-ME and is tested for use in Immunocytochemistry and Western Blotting.

MABF3172-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3172-100UG
      DescriptionAnti-Sup35p-NM Antibody, clone 4A5
      Alternate Names
      • Eukaryotic peptide chain release factor GTP-binding subunit
      • ERF-3
      • ERF3
      • ERF2
      • G1 to S phase transition protein 1
      • Omnipotent suppressor protein 2
      • PSI no more protein 2
      • Polypeptide release factor 3
      • Translation release factor 3
      Background InformationEukaryotic peptide chain release factor GTP-binding subunit (UniProt: P05453; also known as ERF-3, ERF3, ERF2, G1 to S phase transition protein 1, Omnipotent suppressor protein 2, PSI no more protein 2, Polypeptide release factor 3, Translation release factor 3) is encoded by the SUP35 (also known as GST1, PNM2, SAL3, SUF12, SUP2, YD9395.05) gene (Gene ID: 851752) in Saccharomyces cerevisiae (yeast). Sup35 is a GTPase component of the eRF1-eRF3-GTP ternary complex, a complex that mediates translation termination in response to the termination codons UAA, UAG, and UGA. The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. The Saccharomyces cerevisiae (yeast) epigenetic element [PSI+] that has multiple structural variants, is a prion of the translation termination factor subunit Sup35p. The yeast Sup35p and the mammalian PrP prion protein share little structural or functional homology except for a repeat region in the amino terminal domain. Sup35 contains three domains: The N-terminal domain (aa 1-123), which is necessary and sufficient for the [PSI+] propagation; the middle domain (M)(aa 124-253) that interacts with the Hsp104 chaperone through the residues 128-148 and is involved in the liquid-liquid phase separation behavior of Sup35 and imparts stability of the prior during mitosis; and the C-terminal domain (aa 254-685) that acts in translation termination. The N-terminal region also contains a prion-forming domain (aa 1-97) that is unstructured in its native soluble form, but forms beta sheets in its amyloid form. Overexpression of the N-domain is shown to increase the frequency of [PSI+] in yeast. Clone 4A5 specifically recognizes the M-domain of Sup35p and is suitable for Western blotting and immunocytochemistry applications. (Ref.: Dergalev, AA., et al. (2019). Int. J. Mol. Sci. 20(11): 2633; Krammer, C., et al. (2009). Proc. Natl. Acad. Sci. USA. 106 (2); 462-467; Krammer, C., et al. (2008). Prion. 2(3); 99-106).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Sup35p-NM, clone 4A5, Cat. No. MABF3172, is a rat monoclonal antibody that detects of Sup35/HEFE-ME and is tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesTested Applications

      Immunocytochemistry Analysis: A representative lot detected Sup35/HEFE-ME in Immunocytochemistry applications (Krammer, C., et al. (2009). Proc Natl Acad Sci USA. 106(2):462-7).

      Western Blotting Analysis: A representative lot detected Sup35/HEFE-ME in Western Blotting applications (Riemschoss, K., et al. (2019). Life Sci Alliance. 2(4):e201800280).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 19 amino acids from the M-domain of SUP35p protein from Saccharomyces cerevisiae (yeast).
      EpitopeN-terminal half
      Clone4A5
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone 4A5 is a rat monoclonal antibody that detects Eukaryotic peptide chain release factor GTP-binding subunit (SUP35p). It targets an epitope within 19 amino acids from the N-terminal half.
      IsotypeIgG2aκ
      Species Reactivity
      • Yeast
      Species Reactivity NoteSaccharomyces cerevisiae (yeast).
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SUP35
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~45 kDa for HA-tagged Sup35p-NM (M-domain); 76.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from HEK293 cells transfected with HA-tagged Sup35 NM.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Sup35NM (M Domain) in lysate from HEK293 cells transfected with HA-tagged Sup35NM.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3172-100UG 04065271059122