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MABS2297-100UG Anti-TPO Antibody, clone 47

This mouse monoclonal Anti-TPO, clone 47, Cat. No. MABS2297 is tested for use in Western Blotting, Radioimmunoassay and ELISA, for the detection of Thyroid peroxidase (TPO).

MABS2297-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2297-100UG
      DescriptionAnti-TPO Antibody, clone 47
      Alternate Names
      • Thyroid peroxidase
      • MoAb47
      Background InformationThyroid peroxidase (Uniprot: P07202; also known as TPO) is encoded by gene TPO (Gene ID: 7173) in human. It is a single-pass type I membrane protein and has an Extracellular domain (19-846 aa), a Transmembrane domain ( 847-871 aa) and a Cytoplasmic domain (872-933 aa). It is primarily found in the thyroid gland and functions in the iodination of tyrosine residues in thyroglobulin and the formation of phenoxy-ester between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine (T3 and T4). TPO is an integral part of the complex process by which the thyroid gland regulates metabolism and other vital bodily functions. Defects in this gene can cause congenital hypothyroidism, congenital goitre and thyroid hormone organification defect IIA. Moreover, TPO is a common target of autoimmune attacks in various thyroid disorders, such as Hashimoto's thyroiditis and Graves' disease, which can lead to disruptions in hormone synthesis and subsequent health issues.
      (Ref.: Ruf, J., et al. (1989). Endocrinology. 125(3):1211-8. (PMID: 2474433).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationThis mouse monoclonal Anti-TPO, clone 47, Cat. No. MABS2297 is tested for use in Western Blotting, Radioimmunoassay and ELISA, for the detection of Thyroid peroxidase (TPO).
      Key Applications
      • Western Blotting
      • ELISA
      • Radioimmunoassay
      Application NotesTested Applications

      ELISA Analysis: A representative lot detected Thyroid peroxidase (TPO) in ELISA applications (Ruf, J., et al. (1989). Endocrinology. 125(3):1211-8).

      Radioimmunoassay: A representative lot detected Thyroid peroxidase (TPO) in Radioimmunoassay applications (Ruf, J., et al. (1989). Endocrinology. 125(3):1211-8).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHuman thyroid peroxidase (hTPO) purified from thyroid microsomes by immunoaffinity chromatography.
      EpitopeUnknown
      Clone47
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 47 is a mouse monoclonal antibody that specifically detects Thyroid peroxidase.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TPO
      Purification MethodProtein G purified
      UniProt Number
      Molecular WeightTarget molecular weight ~120 kDa observed; 102.96 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human thyroid tissue extract.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Thyroid peroxidase (TPO) in human thyroid tissue extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2297-100UG 04065271270060