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OP129 Anti-TRF2 Mouse mAb (4A794)

Anti-TRF2 Mouse mAb (4A794) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-TTAGGG Repeat Binding Factor 2

OP129
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~66 kDa (doublet) TRF2 protein in HL60 cells.
      Catalogue NumberOP129
      Brand Family Calbiochem®
      SynonymsAnti-TTAGGG Repeat Binding Factor 2
      Application Data
      Detection of human TRF2 by immunoprecipitation, followed by immunoblotting. Sample: Whole cell lysate from 1x106 HL-60 cells. Antibodies for IP: Anti-TRF2 Mouse mAb (4A794) (2 µg) (lane 1), control mouse IgG (lane 2), purified goat-anti-TRF2 (lane 3) or pre-immune goat IgG (lane 4). Primary antibody for IB: Anti-TRF2 Mouse mAb (4A794) (Cat. No. OP129) (2 µg/ml). Detection: chemiluminescence.
      References
      ReferencesKarlseder, J., et al. 1999. Science 283, 1321.
      Van Steensel, B., et al. 1998. Cell 92, 401.
      Broccoli, D., et al. 1997. Nature Genetics 17, 231.
      Product Information
      FormLiquid
      FormulationIn PBS, 0.05% BSA.
      Positive controlHL-60 cells or most human cell lines
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Application NotesImmunoblotting (2 µg/ml)
      Immunocytochemistry (5 µg/ml)
      Immunoprecipitation (2 µg/1 million cells)
      Application CommentsThe antibody recognizes distinct doublet bands of approximate size 66 kD corresponding to the molecular size of huTRF2 by western blot analysis against lysates from most human cell lines. The antibody also gives nuclear staining of cells by immunocytochemistry.
      Biological Information
      Immunogena recombinant protein consisting of full-length human TRF2 fused to a His•Tag® sequence, expressed in baculovirus
      ImmunogenHuman
      Clone4A794
      HostMouse
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OP129 0

      Documentation

      Anti-TRF2 Mouse mAb (4A794) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-TRF2 Mouse mAb (4A794) Certificates of Analysis

      TitleLot Number
      OP129

      References

      Reference overview
      Karlseder, J., et al. 1999. Science 283, 1321.
      Van Steensel, B., et al. 1998. Cell 92, 401.
      Broccoli, D., et al. 1997. Nature Genetics 17, 231.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-September-2008 RFH
      SynonymsAnti-TTAGGG Repeat Binding Factor 2
      ApplicationImmunoblotting (2 µg/ml)
      Immunocytochemistry (5 µg/ml)
      Immunoprecipitation (2 µg/1 million cells)
      Application Data
      Detection of human TRF2 by immunoprecipitation, followed by immunoblotting. Sample: Whole cell lysate from 1x106 HL-60 cells. Antibodies for IP: Anti-TRF2 Mouse mAb (4A794) (2 µg) (lane 1), control mouse IgG (lane 2), purified goat-anti-TRF2 (lane 3) or pre-immune goat IgG (lane 4). Primary antibody for IB: Anti-TRF2 Mouse mAb (4A794) (Cat. No. OP129) (2 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~66 kDa huTRF2 protein.
      BackgroundTRF2 is a ubiquitously expressed protein that is implicated in the control of telomere length. TRF2, like TRF1 contains a Myb-related DNA binding motif. It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes. TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini. The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells. Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types.
      HostMouse
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of full-length human TRF2 fused to a His•Tag® sequence, expressed in baculovirus
      Clone4A794
      IsotypeIgG
      Specieshuman
      Positive controlHL-60 cells or most human cell lines
      FormLiquid
      FormulationIn PBS, 0.05% BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThe antibody recognizes distinct doublet bands of approximate size 66 kD corresponding to the molecular size of huTRF2 by western blot analysis against lysates from most human cell lines. The antibody also gives nuclear staining of cells by immunocytochemistry.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesKarlseder, J., et al. 1999. Science 283, 1321.
      Van Steensel, B., et al. 1998. Cell 92, 401.
      Broccoli, D., et al. 1997. Nature Genetics 17, 231.