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ST1659 Anti-YARS Mouse mAb (2F3)

Anti-YARS Mouse mAb (2F3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Tyrosyl-tRNA Synthetics

ST1659
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~50-52 kDa YARS protein in HeLa and NIH3T3 cells.
      Catalogue NumberST1659
      Brand Family Calbiochem®
      SynonymsAnti-Tyrosyl-tRNA Synthetics
      Application Data
      Detection of human YARS by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-YARS Mouse mAb (2F3) (Cat. No. ST1659) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse YARS by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-YARS Mouse mAb (2F3) (Cat. No. ST1659) (1-5 µg/ml). Detection: chemiluminescence.
      References
      ReferencesStorkebaum, E., et al. 2009. Proc. Natl. Acad. Sci. U S A 106, 11782.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHeLa cells, NIH3T3 cells
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1-5 µg/ml)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length recombinant human YARS (aa 1-529), expressed as a GST fusion protein
      Clone2F3
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1659 0

      Documentation

      References

      Reference overview
      Storkebaum, E., et al. 2009. Proc. Natl. Acad. Sci. U S A 106, 11782.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-November-2010 RFH
      SynonymsAnti-Tyrosyl-tRNA Synthetics
      ApplicationImmunoblotting (1-5 µg/ml)
      Application Data
      Detection of human YARS by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-YARS Mouse mAb (2F3) (Cat. No. ST1659) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse YARS by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-YARS Mouse mAb (2F3) (Cat. No. ST1659) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~50-52 kDa YARS protein.
      BackgroundAminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.
      HostMouse
      ImmunogenFull-length recombinant human YARS (aa 1-529), expressed as a GST fusion protein
      Clone2F3
      IsotypeIgG₁
      Specieshuman, mouse
      Positive controlHeLa cells, NIH3T3 cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesStorkebaum, E., et al. 2009. Proc. Natl. Acad. Sci. U S A 106, 11782.