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ABC441-25UL Anti-ZKSCAN3 (Zf47)

Anti-ZKSCAN3 (Zf47), Cat. No. ABC441, is a rabbit polyclonal antibody that detects Zinc finger protein with KRAB and SCAN domains 3 and has been tested for use in Immunocytochemistry and Western Blotting.

Research Category: Apoptosis & Cancer Gene Symbol: ZKSCAN3, ZFP47, ZNF306, ZNF309, ZSCAN13 UniProt Number: Q9BRR0
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ABC441-25UL
25 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC441-25UL
      DescriptionAnti-ZKSCAN3 (Zf47)
      Alternate Names
      • Zinc finger protein with KRAB and SCAN domains 3
      • Zinc finger and SCAN domain-containing protein 13
      • Zinc finger protein 306
      • Zinc finger protein 309
      • Zinc finger protein 47 homolog
      • Zf47
      • Zfp-47
      Background InformationZinc finger protein with KRAB and SCAN domains 3 (UniProt: Q9BRR0; also known as Zinc finger and SCAN domain-containing protein 13, Zinc finger protein 306, Zinc finger protein 309, Zinc finger protein 47 homolog, Zf47, Zfp-47) is encoded by the ZKSCAN3 (also known as ZFP47, ZNF306, ZNF309, ZSCAN13) gene (Gene ID: 80317) in human. Zf47 is a member of the krueppel C2H2-type zinc-finger protein family. Two isoforms of Zf47 have been described that are produced by alternative splicing. It contains 7 C2H2 type zinc-fingers. It serves as a transcriptional factor that binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3' and acts as a repressor of autophagy. It is shown to specifically repress expression of genes involved in autophagy and lysosome biogenesis/function such as MAP1LC3B, ULK1 or WIPI2. It is mainly localized in the nucleus, however, under conditions of starvation, it translocates to the cytoplasm, allowing expression of target genes involved in autophagy and lysosome biogenesis/function. Overexpression of Zf47 is observed in various tumors, including multiple myeloma, colorectal and prostate cancers.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-ZKSCAN3 (Zf47), Cat. No. ABC441, is a rabbit polyclonal antibody that detects Zinc finger protein with KRAB and SCAN domains 3 and has been tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ZKSCAN3 (Zf47) with KRAB and SCAN domains 3 in HeLa and A431 cell lines.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 12 amino acids from the N-terminal half of human Zinc finger protein with KRAB and SCAN domains 3. The immunogen sequence is conserved in both isoforms, 1 and 2.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Zinc finger protein with KRAB and SCAN domains 3 in human cells. It targets an epitope within 12 amino acids from the N-terminal half.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ZKSCAN3
      • ZFP47
      • ZNF306
      • ZNF309
      • ZSCAN13
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~65 kDa observed; 60.64 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ZKSCAN3 (Zf47) with KRAB and SCAN domains 3 in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC441-25UL 04054839441073

      Documentation