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218828 Caspase-1 Substrate XV, Cell-Permeable, Fluorogenic

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Nα-5-Tetramethylrhodaminyl-YVADAC(S-acrylodan)-OH, TMR-YVADAC(AD)

218828
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Please refer to our complete listing of caspase substrates for possible alternatives.






      A cell-permeable, internally-quenched fluorogenic peptide substrate that is useful for detecting in vivo caspase-1-like protease activity in living cells or tissues. Efficiently cleaved by caspase-1 in vitro at the peptide bond between Asp and Ala residues (kcat/Km ~ 8.5 x 10-2 µM-1s-1) with an increase in fluorescence intensity (~525 nm) when excited at 380 nm. Both time-dependent and sub-cellular localization of caspase-1-like activity could be visualized in glutamate-induced rat hippocampal cell death.
      Catalogue Number218828
      Brand Family Calbiochem®
      SynonymsNα-5-Tetramethylrhodaminyl-YVADAC(S-acrylodan)-OH, TMR-YVADAC(AD)
      References
      ReferencesNishii, W., et al. 2002. FEBS Lett. 518, 149.
      Product Information
      FormRed-purple lyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₆₇H₇₅N₉O₁₅S
      Chemical formulaC₆₇H₇₅N₉O₁₅S
      Applications
      Biological Information
      Purity≥90% by HPLC
      Physicochemical Information
      Peptide SequenceTAMRA(5)-Tyr-Val-Ala-Asp-Ala-Cys(S-acrylodan)-OH
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsUnstable in solution; reconstitute just prior to use.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      218828 0

      Documentation

      Caspase-1 Substrate XV, Cell-Permeable, Fluorogenic Certificates of Analysis

      TitleLot Number
      218828

      References

      Reference overview
      Nishii, W., et al. 2002. FEBS Lett. 518, 149.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-June-2008 RFH
      SynonymsNα-5-Tetramethylrhodaminyl-YVADAC(S-acrylodan)-OH, TMR-YVADAC(AD)
      DescriptionA cell-permeable, internally-quenched fluorogenic peptide substrate that is useful for detecting caspase-1-like protease activity in living cells or cultured tissues. Efficiently cleaved by caspase-1 in vitro at the peptide bond between Asp and Ala residues (kcat/Km ~8.5 x 10-2µM-1s-1) with an increase in fluorescence intensity at ~520 nm. Excitation max: ~380 nm; emission max: ~520 nm.
      FormRed-purple lyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₆₇H₇₅N₉O₁₅S
      Peptide SequenceTAMRA(5)-Tyr-Val-Ala-Asp-Ala-Cys(S-acrylodan)-OH
      Purity≥90% by HPLC
      SolubilityDMSO (5 mg/ml)
      Storage Protect from moisture
      Protect from light
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsUnstable in solution; reconstitute just prior to use.
      Toxicity Standard Handling
      ReferencesNishii, W., et al. 2002. FEBS Lett. 518, 149.