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FC010-5MG Sigma-AldrichHuman Plasma Fibronectin Purified Protein

Human Plasma Fibronectin Purified Protein MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

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Data Sheet

Key Applications: Cell Culture

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FC010-5MG

5 mg 5 x 1 mg

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Key Spec Table

Key Applications
CULT

Description
Catalogue Number	FC010-5MG
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Human Plasma Fibronectin Purified Protein
Overview	Human fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plastic ware, glassware, and microcarrier beads.

References

Product Information
Presentation	Liquid in 50 mM tris-buffered saline (TBS), pH 7.5, containing no preservatives.
Quality Level	MQ100

Applications
Key Applications	Cell Culture
Application Notes	Immunochemical standard Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts Suggested Procedure for Coating Cell Cultureware 1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (μg/mL) of HFN. Recommended amount is 2-10 μg/cm2. 2.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area. 3. Introduce the proper CO2 atmosphere, if required. 4. Add the calculated amount of HFN to each culture vessel. 5. Allow HFN to adsorb to the surface of the vessel for 5-20 minutes. 6. Remove residual balanced salt solution before proceeding with standard cell culture procedures

Applications

Key Applications

Cell Culture

Application Notes

Immunochemical standard

Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts

Suggested Procedure for Coating Cell Cultureware

1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (μg/mL) of HFN. Recommended amount is 2-10 μg/cm2.

2.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.

3. Introduce the proper CO2 atmosphere, if required.

4. Add the calculated amount of HFN to each culture vessel.

5. Allow HFN to adsorb to the surface of the vessel for 5-20 minutes.

6. Remove residual balanced salt solution before proceeding with standard cell culture procedures

Biological Information
Concentration	1 mg/mL
Purity	Approximately 95%, as determined by SDS-PAGE. A double band of 220 kDa is present under reduced conditions. HFN is purified by affinity chromatography on gelatin agarose, followed by chromatography on heparin-agarose.
Source	Human donor plasma.
Entrez Gene Number	NM_002026.2 NM_054034.2 NM_212474.1 NM_212475.1 NM_212476.1 NM_212478.1 NM_212482.1
Entrez Gene Summary	This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
Gene Symbol	FN1 DKFZp686O13149 DKFZp686H0342 DKFZp686I1370 MSF FN CIG DKFZp686F10164 LETS FINC
UniProt Number	P02751
UniProt Summary	FUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity). SIZE: 2386 amino acids; 262607 Da SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1. SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. PTM: Sulfated. SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.
Stem Cell Type	Human Embryonic Stem Cells Mesenchymal Stem Cells Neural Stem Cells Hematopoietic Stem Cells Epithelial Cells Pancreatic Stem Cells Induced Pluripotent Stem Cells

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at 2-8°C for up to 6 months from date of receipt. Do not freeze.

Packaging Information
Material Size	5 mg
Material Package	5 x 1 mg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
FC010-5MG	04053252331183

Documentation

Human Plasma Fibronectin Purified Protein Certificates of Analysis

Title	Lot Number
HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2147051	2147051
HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2382597	2382597
HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2445845	2445845
HUMAN PLASMA FIBRONECTIN - 3172081	3172081
HUMAN PLASMA FIBRONECTIN - 3179932	3179932
HUMAN PLASMA FIBRONECTIN - 3202373	3202373
HUMAN PLASMA FIBRONECTIN - 3221086	3221086
HUMAN PLASMA FIBRONECTIN - 3256639	3256639
HUMAN PLASMA FIBRONECTIN - 3279313	3279313
HUMAN PLASMA FIBRONECTIN - 3308854	3308854

References

Reference overview	Pub Med ID
Comparative studies on amniotic fluid and plasma fibronectins Ruoslahti E. et al. Biochem. J, 193:295-299 (1981) 1981	7305927

Brochure

Title
Human Stem Cell Systems

Data Sheet

Title
Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

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FC010-5MG Sigma-AldrichHuman Plasma Fibronectin Purified Protein

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