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539685 PKCι, GST-Fusion, Human, Recombinant, S. frugiperda

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Protein Kinase C

539685
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Full-length, recombinant, human PKCι (accession number = NM_002740) fused at the N-terminus to GST and expressed in S. frugiperda insect cells using a baculovirus expression system. PKCι is a member of the protein kinase C family that plays an important role in neuralonal cell apoptosis and neuronal degeneration in Alzheimer's disease.
      Catalogue Number539685
      Brand Family Calbiochem®
      SynonymsProtein Kinase C
      Application Data
      Increasing concentrations of PKCι were assayed as outlined above.
      References
      ReferencesGustrafson, W.C., et al. 2004. J. Biol. Chem. 279, 9400.
      Zhang, J., et al. 2004. J. Biol. Chem. 279, 22118.
      Product Information
      Unit of DefinitionKinase activity is measured as the molar amount of phosphate incorporated into CREBtide using a concentration of 50 µM ATP (0.83 µCi/assay), 30°C.
      FormLiquid
      Formulation150 mM NaCl, 50 mM Tris-HCl, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM EGTA, 0.1 mM PMSF, 25% glycerol, pH 7.5.
      Applications
      Biological Information
      Primary TargetPKCι
      Purity≥90% by SDS-PAGE
      Specific Activity≥565 nmol/min/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      539685 0

      Documentation

      PKCι, GST-Fusion, Human, Recombinant, S. frugiperda Certificates of Analysis

      TitleLot Number
      539685

      References

      Reference overview
      Gustrafson, W.C., et al. 2004. J. Biol. Chem. 279, 9400.
      Zhang, J., et al. 2004. J. Biol. Chem. 279, 22118.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-September-2008 RFH
      SynonymsProtein Kinase C
      Application Data
      Increasing concentrations of PKCι were assayed as outlined above.
      DescriptionFull-length, recombinant, human PKCι (accession number = NM_002740) fused at the N-terminus to GST and expressed in S. frugiperda insect cells using a baculovirus expression system. PKCι is a member of the protein kinase C family that plays an important role in neuralonal cell apoptosis and neuronal degeneration in Alzheimer's disease.
      FormLiquid
      Formulation150 mM NaCl, 50 mM Tris-HCl, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM EGTA, 0.1 mM PMSF, 25% glycerol, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      Kinase assay conditions: Dilute purified PKCι in assay dilution buffer (4 mM MOPS, 4 mM MgCl2, 2.5 mM β-glycerophosphate, 1 mM EGTA, 0.4 mM EDTA, 50 µM DTT, pH 7.2) to the desired concentration. Mix 7.5 µl diluted enzyme, 10 µl CREBtide substrate (KRREILSRRPSYR, 1 mg/ml stock), and 2.5 µl lipid activators (0.5 mg/ml phosphatidylserine and 0.05 mg/ml diacylglycerol in 25 mM β-glycerophosphate, 20 mM MOPS, 1 mM sodium orthovanadate, 1 mM dithioreitol, 1 mM CaCl2, pH 7.2; sonicate for 1 min prior to use) and incubate for 5 min at 30°C. Add 5 µl ATP mixture (250 µM ATP, 0.16 µCi/µl in 4X assay dilution buffer) and incubate for 15 min at 30°C. Terminate the reaction by spotting 20 µl of the reaction mixture onto a phosphocellulose P81 paper, dry, and wash several times with 1% phosphoric acid prior to counting in the presence of scintillation fluid.
      Purity≥90% by SDS-PAGE
      Specific activity≥565 nmol/min/mg protein
      Unit definitionKinase activity is measured as the molar amount of phosphate incorporated into CREBtide using a concentration of 50 µM ATP (0.83 µCi/assay), 30°C.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesGustrafson, W.C., et al. 2004. J. Biol. Chem. 279, 9400.
      Zhang, J., et al. 2004. J. Biol. Chem. 279, 22118.