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656000 PhosphoDetect™ Anti-Tyk2 (pTyr1054/1055) Rabbit pAb

This PhosphoDetect™ Anti-Tyk2 (pTyr1054/1055) Rabbit pAb is validated for use in Immunoblotting for the detection of Tyk2 (pTyr1054/1055).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
656000
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.

      Recognizes Tyk2 phosphorylated at Tyr1054/1055.
      Catalogue Number656000
      Brand Family Calbiochem®
      Application Data
      Detection of human Tyk2, phosphorylated on Tyr1054/1055, by immunoblotting. Samples: HeLa cell extracts untreated or treated with IFN-alpha. Primary antibody: PhosphoDetect™ Anti-Tyk2 (pTyr1054/1055) Rabbit pAb (Cat. No. 656000) (1:1000). Detection: chemiluminescence.
      References
      ReferencesGauzzi, M.C., et al. 1996. J. Biol. Chem. 271, 20494.
      Colamonici, O.R., et al. 1994. J. Biol. Chem. 269, 3518.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000, chemiluminescence)
      Application CommentsTo reduce basal levels of Tyk2 phosphorylation, incubate cells in low-serum (0.5%) medium for 2 days. Change to fresh serum-free medium immediately before treatment. Does not recognize nonphosphorylated Tyk2. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids surrounding the Tyr1054/1055 phosphorylation sites of human Tyk2, conjugated to KLH
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      656000 0

      Documentation

      PhosphoDetect™ Anti-Tyk2 (pTyr1054/1055) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-Tyk2 (pTyr1054/1055) Rabbit pAb Certificates of Analysis

      TitleLot Number
      656000

      References

      Reference overview
      Gauzzi, M.C., et al. 1996. J. Biol. Chem. 271, 20494.
      Colamonici, O.R., et al. 1994. J. Biol. Chem. 269, 3518.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-August-2007 RFH
      ApplicationImmunoblotting (1:1000, chemiluminescence)
      Application Data
      Detection of human Tyk2, phosphorylated on Tyr1054/1055, by immunoblotting. Samples: HeLa cell extracts untreated or treated with IFN-alpha. Primary antibody: PhosphoDetect™ Anti-Tyk2 (pTyr1054/1055) Rabbit pAb (Cat. No. 656000) (1:1000). Detection: chemiluminescence.
      DescriptionProtein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes Tyk2 phosphorylated at Tyr1054/1055.
      BackgroundTyk2, a member of the Janus Kinase (JAK) family of kinases, is associated with the IFN-α/β receptor. Upon ligand binding, Tyk2 is activated by phosphorylation on Tyr1054 and/or Tyr1055, initiating the IFN-α/β signaling cascade.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids surrounding the Tyr1054/1055 phosphorylation sites of human Tyk2, conjugated to KLH
      IsotypeIgG
      Specieshuman
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsTo reduce basal levels of Tyk2 phosphorylation, incubate cells in low-serum (0.5%) medium for 2 days. Change to fresh serum-free medium immediately before treatment. Does not recognize nonphosphorylated Tyk2. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesGauzzi, M.C., et al. 1996. J. Biol. Chem. 271, 20494.
      Colamonici, O.R., et al. 1994. J. Biol. Chem. 269, 3518.