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TR-1008-G Sodium Butyrate Solution

This reagent enhances the transient expression of plasmids containing the SV40 early promoter in transfected cells.

Sodium Butyrate Solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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TR-1008-G
1 mL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberTR-1008-G
      Trade Name
      • Specialty Media
      DescriptionSodium Butyrate Solution
      OverviewThis reagent enhances the transient expression of plasmids containing the SV40 early promoter in transfected cells. Available as a 500mM stock solution (1mL). A complete protocol is included with shipment.
      References
      Product Information
      Quality LevelMQ200
      Applications
      ApplicationThis reagent enhances the transient expression of plasmids containing the SV40 early promoter in transfected cells.
      Application NotesSodium butyrate can act to increase the efficiency of transfection and expression for both transient and stable transfections. It increases enhancer-dependant transcription of inserts driven by the SV40 early promoter. Vectors of this type include any of the pSV and pSV2 plasmids. Treatment of transfected cells with sodium butyrate can result in: a 2-3 fold increase in the number of cells able to express the insert; a 10-30 fold increase in the number of stable transfected colonies; and cells treated with sodium butyrate at the time of transfection can be re-induced by a second treatment weeks after the initial treatment and yield a 4-7 fold increase in transcription of the SV40 early promoter driven insert.



      This treatment can be used on cells transfected by the Calcium Phosphate, DEAE Dextran and electroportation method.
      Biological Information
      Media FormLiquid
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2-8°C for 2 years.
      Packaging Information
      Material Size1 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      TR-1008-G 04053252504877