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Merck

1.25193

Cellvento® 4HEK Liquid

Chemically defined, sterile; sterile-filtered, suitable (for HEK293 cell culture), liquid

Synonym(s):

AAV gene therapy medium, Cellvento® 4HEK, Cellvento® 4HEK Liquid, Cellvento® 4HEK liquid medium, HEK293 AAV production medium, HEK293 cell culture medium, HEK293 suspension medium, liquid HEK medium

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About This Item

UNSPSC Code:
12352200
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Quality Segment

biological source

non-animal source

description

shipped with cooling pack.

Liquid medium must be supplemented with 6–10 mM L-Glutamine.

sterility

sterile; sterile-filtered

product line

Cellvento®

form

liquid

shelf life

12 mo. (For 20 L bag), 24 mo. (For 1 L )

technique(s)

cell culture | mammalian: suitable (suspension)

application(s)

cell culture
pharma/biopharma processes

compatibility

for use with multiple HEK293 suspension cell lines; compatible with commonly used transfection reagents (PEI & Mirus Bio)

components

L-glutamine: no

shipped in

cooler

storage temp.

2-8°C

General description

Cellvento® 4HEK Medium is a chemically defined medium optimized for adeno-associated virus (AAV) production in multiple human embryonic kidney (HEK) 293 lineages. Cellvento® 4HEK Medium has been streamlined and cell culture media components incorporated to increase media stability and reduce protein oxidation. The formulation is animal component free.

Application

  • Cellvento® 4HEK Medium can be used as amplification and production medium for gene therapy applications.
  • This medium enables low doubling time and high viable cell densities with multiple HEK293 cell lines.

Features and Benefits

  • Cellvento®4HEK Medium is compatible with commonly used transfection reagents like PEI, supports AAV production and facilitates high titers of full capsids in various HEK293 lineages.
  • This easy-to-use medium can be used for all stages like growth, transfection, and production.

Preparation Note

Formulated to contain 30.8 grams of powder per liter of medium.

Legal Information

CELLVENTO is a registered trademark of Merck KGaA, Darmstadt, Germany


Storage Class

12 - Non Combustible Liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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