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Merck

1.25195

Cellvento® 4HEK Powder

Chemically defined medium, suitable (for HEK293 cell culture), dry powder

Synonym(s):

AAV gene therapy medium, Cellvento® 4HEK, Cellvento® 4HEK Powder, Cellvento® 4HEK dry powder medium, HEK293 AAV production medium, HEK293 cell culture medium, HEK293 suspension medium, chemically defined HEK293 medium

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About This Item

UNSPSC Code:
41106213
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Quality Segment

biological source

non-animal source

description

shipped with cooling pack.

Dry powder medium must be supplemented with 2.5 g/L Sodium Bicarbonate. Once hydrated, medium must be supplemented with 6-10 mM L-Glutamine

product line

Cellvento®

form

dry powder

shelf life

24 mo.

technique(s)

cell culture | mammalian: suitable

suitability

suitable (for HEK293 cell culture)

application(s)

cell culture
pharma/biopharma processes

compatibility

for use with multiple HEK293 suspension cell lines; compatible with commonly used transfection reagents (PEI & Mirus Bio)

components

L-glutamine: no

shipped in

cooler

storage temp.

2-8°C

General description

Cellvento® 4HEK Medium is a chemically defined medium optimized for adeno-associated virus (AAV) production in multiple human embryonic kidney (HEK) 293 lineages. Cellvento® 4HEK Medium has been streamlined and cell culture media components incorporated to increase media stability and reduce protein oxidation. The formulation is animal component free.

Application

  • Used as an amplification and production medium for gene therapy applications, especially for adeno-associated virus (AAV) production in HEK293 cell lines.
  • Suitable for all stages of the AAV production process, including cell expansion, transfection, and virus production

Features and Benefits

  • Enables rapid cell growth and high viable cell densities across multiple HEK293 cell variants.
  • Compatible with commonly used transfection reagents such as PEI, supporting efficient transfection protocols.

Preparation Note

Dry powder medium must be supplemented with 2.5 g/LSodium Bicarbonate. Once hydrated, medium must be supplemented with 6-10 mML-Glutamine.

Legal Information

CELLVENTO is a registered trademark of Merck KGaA, Darmstadt, Germany


Pictograms

Exclamation mark

Signal Word

Warning

Hazard Codes

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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