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07-641

Anti-MAP2K1/MEK1 Antibody

Upstate, rabbit polyclonal

Synonym(s):

Anti-CFC3, Anti-MAPKK1, Anti-MEK1, Anti-MEL, Anti-MKK1, Anti-PRKMK1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
mouse, rat, human
Application:
IP, WB
Citations:
37
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Product Name

Anti-MEK1 Antibody, Upstate®, from rabbit

biological source

rabbit

Quality Segment

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat, human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG

NCBI accession no.

NM_002755

UniProt accession no.

Q02750

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... MAP2K1(5604)

General description

45kDa

Immunogen

Peptide corresponding to amino acids 2-18 (PKKKPTPIQLNPAPDGS) of human MEK1.

Application

Research Category
Signaling
Research Sub Category
MAP Kinases
This Anti-MEK1 Antibody is validated for use in IP, WB for the detection of MEK1.

Biochem/physiol Actions

MEK1
Predicted to cross-react with rabbit based on sequence homology.

Physical form

70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide) and 30% glycerol.
Format: Purified
Protein A purified

Preparation Note

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.
Routinely evaluated by immunoblot on RIPA lysates from A431 and 3T3/A31 cells.

Other Notes

Replaces: 04-376

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

07-641:

jan

Certificates of Analysis (COA)

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Global Trade Item Number

SKUGTIN
07-64104053252621413