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Merck

1.04119

Osmium(VIII) oxide

for microscopy

Synonym(s):

Osmium(VIII) oxide

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About This Item

Empirical Formula (Hill Notation):
O4Os
CAS Number:
20816-12-0
Molecular Weight:
254.23
UNSPSC Code:
12352303
MDL number:
MFCD00011150

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Product Name

Osmium(VIII) oxide, for microscopy

SMILES string

[Os](=O)(=O)(=O)=O

InChI

1S/4O.Os

InChI key

VUVGYHUDAICLFK-UHFFFAOYSA-N

vapor pressure

10 hPa ( 20 °C)

potency

15 mg/kg LD50, oral (Rat)

color

yellow

pH

6 (20 °C, 25 g/L in H2O)

bp

130 °C/1013 hPa

mp

40 °C (decomposition)

solubility

65 g/L

density

4.91 g/cm3 at 20 °C

bulk density

1500 kg/m3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

Quality Level

200

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Analysis Note

Identity (Raman-spectrum): conforms

Application

for microscopy

General description

Osmium tetroxide for microscopy can be used to visualize unsaturated lipids. A part of the lipids is immediately blackened by the influence of osmic acid solution. The immediate black coloration results from the -C=C- double bond of the unsaturated fatty acid. The fatty acids that are made brownish at first can be blackened by treatment with 60-70% alcohol. For electron microscopy or semithin sectioning the glutardialdehyde-fixed specimens are post-fixed with osmium(VIII) oxide. Lipid inclusions appear black on a yellow to brown background.

pictograms

CorrosionSkull and crossbones
GHS05,GHS06

signalword

Danger

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Eye Dam. 1 - Skin Corr. 1B

Storage Class

6.1B - Non-combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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