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Merck

12-309

HeLa Nuclear Extract

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About This Item

UNSPSC Code:
41105330
NACRES:
NA.41
eCl@ss:
32011807
Biological source:
human
Manufacturer/tradename:
Upstate®

Key Documents

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biological source

human

form

liquid

manufacturer/tradename

Upstate®

concentration

2.0 mg/mL

technique(s)

western blot: suitable

shipped in

dry ice

Quality Level

100

General description

Nuclear extract prepared from human HeLa cells using a modified protocol of Dignam, et al., which contains a variety of DNA binding proteins and transcription factors.

Biochem/physiol Actions

HeLa

Physical form

RIPA sample buffer containing inhibitors. Frozen solution.

Preparation Note

Stable for 6 months at -70°C from date of shipment.

Analysis Note

Routinely evaluated as a positive control for many of Upstates antibodies.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Class I Designated Chemical Substances

prtr

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

12-309:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tabetha Sundin et al.
Biochimie, 94(12), 2639-2648 (2012-08-21)
Isoprenoids are recognized for their ability to suppress carcinogenic processes in vivo and in vitro. We previously established that the isoprenoid, perillyl alcohol, acted mechanistically on translation of specific proteins through modulation of mechanistic target of rapamycin (mTOR) signaling. Telomerase-the
J D Dignam et al.
Nucleic acids research, 11(5), 1475-1489 (1983-03-11)
We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major

Global Trade Item Number

SKUGTIN
12-30904053252283925

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