Skip to Content
Merck

14-279

Akt1/PKBα Protein, inactive, 50 g

Unactive, N-terminal His6-tagged recombinant full-length human Akt1, for use in Kinase Assays.

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

UNSPSC Code:
12352202
NACRES:
NA.26
eCl@ss:
32160405
Biological source:
human
Recombinant:
expressed in baculovirus infected Sf21 cells
Mol wt:
Mw 59 kDa

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

human

recombinant

expressed in baculovirus infected Sf21 cells

mol wt

Mw 59 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

NM_001014431.1, NM_001014432.1, NM_005163.2

UniProt accession no.

P31749

shipped in

dry ice

Quality Level

100

General description

N-terminal His6-tagged recombinant full-length human Akt1

Analysis Note

routinely evaluated by phosphorylation of Crosstide

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Deleterious substance

pdsc

14-279:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Jorge Vera et al.
eLife, 4 (2015-11-28)
The PP2A phosphatase is often inactivated in cancer and is considered as a tumour suppressor. A new pathway controlling PP2A activity in mitosis has been recently described. This pathway includes the Greatwall (GWL) kinase and its substrates endosulfines. At mitotic
Hiroshi Senoo et al.
Nature cell biology, 21(7), 867-878 (2019-07-03)
mTORC2 plays critical roles in metabolism, cell survival and actin cytoskeletal dynamics through the phosphorylation of AKT. Despite its importance to biology and medicine, it is unclear how mTORC2-mediated AKT phosphorylation is controlled. Here, we identify an unforeseen principle by
Dos D Sarbassov et al.
Molecular cell, 22(2), 159-168 (2006-04-11)
The drug rapamycin has important uses in oncology, cardiology, and transplantation medicine, but its clinically relevant molecular effects are not understood. When bound to FKBP12, rapamycin interacts with and inhibits the kinase activity of a multiprotein complex composed of mTOR
Chia-Chen Chen et al.
Developmental cell, 18(4), 592-604 (2010-04-24)
FoxO transcription factors and TORC1 are conserved downstream effectors of Akt. Here, we unraveled regulatory circuits underlying the interplay between Akt, FoxO, and mTOR. Activated FoxO1 inhibits mTORC1 by TSC2-dependent and TSC2-independent mechanisms. First, FoxO1 induces Sestrin3 (Sesn3) gene expression.
Stoichiometry of site-specific protein phosphorylation estimated with phosphopeptide-specific antibodies.
Miinea, Cristinel P and Lienhard, Gustav E
Biotechniques, 34, 828-831 (2003)
View All Related Papers

Global Trade Item Number

SKUGTIN
14-27904053252624872

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service