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Merck

345810

G 418 Sulfate, Cell Culture Tested

G418 also known as Geneticin is an aminoglycoside antibiotic related to Gentamicin. Used as a selective agent in transfection of eukaryotic cells. Has highest potency ≥730 µg/mg and purity ≥98%.

Synonym(s):

G 418 Sulfate, Cell Culture Tested, G418, G 418, NMD Inhibitor I

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About This Item

Empirical Formula (Hill Notation):
C20H40N4O10 · 2H2SO4
CAS Number:
108321-42-2
Molecular Weight:
692.71
UNSPSC Code:
12352200
MDL number:
MFCD00058314
Assay:
≥98% (TLC)
Form:
powder
Quality level:
100
Storage condition:
OK to freeze

Key Documents

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InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI key

UHEPSJJJMTWUCP-NKCAIAFTSA-N

assay

≥98% (TLC)

form

powder

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤1.0% Ammonia

color

white

solubility

water: 100 mg/mL, clear, colorless

cation traces

heavy metals: ≤100 ppm

shipped in

ambient

storage temp.

10-30°C

Quality Level

100

General description

Also available as a sterile-filtered aqueous solution (Cat. No. 345812), a sterile filtered solution in 100 mM HEPES (Cat. No. 508940), and a sterile-filtered solution PBS (Cat. No. 509227).

Preparation Note

Following reconstitution, sterilize by filtration through a 0.22 µm or 0.45 µm pore size filter, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Sterile stock solutions are stable for up to 1 year at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

345810-25GM: + US2345810-250: + 345810-500MG: + 345810-250GM: + 345810-2.5KG: + 345810-1GM: + 345810-5GM: + 345810-250MG: + 345810-100GM: + US2345810-5GM: + US2345810-1GM: + 345810-MG:

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Wilfried Weber et al.
Methods in molecular biology (Clifton, N.J.), 267, 451-466 (2004-07-23)
Inducible expression of desired transgenes in mammalian cells and animals is a current priority in basic and applied research, biopharmaceutical manufacturing, gene therapy, and tissue engineering, as well as in drug discovery. Among the most prominent human-compatible transgene control technologies
Transgene control engineering in mammalian cells.
Beat P Kramer et al.
Methods in molecular biology (Clifton, N.J.), 308, 123-143 (2005-08-06)
Qin Zhou et al.
Biotechnology journal, 16(9), e2000623-e2000623 (2021-05-31)
Chinese hamster ovary (CHO) cells with a high viable cell density (VCD), resilience to culture stress, and the capacity to continuously express recombinant proteins are highly desirable. Phosphatase and tension homology deleted on chromosome ten (PTEN) functions as a key
Johannes H Hegemann et al.
Methods in molecular biology (Clifton, N.J.), 765, 189-206 (2011-08-05)
Gene inactivation is an essential step in the molecular dissection of gene function. In the yeast Saccharomyces cerevisiae, many tools for gene disruption are available. Gene disruption cassettes comprising completely heterologous marker genes flanked by short DNA segments homologous to
Carlos Barrera-Avalos et al.
iScience, 24(12), 103520-103520 (2021-12-25)
T cell activation requires the processing and presentation of antigenic peptides in the context of a major histocompatibility complex (MHC complex). Cross-dressing is a non-conventional antigen presentation mechanism, involving the transfer of preformed peptide/MHC complexes from whole cells, such as
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Global Trade Item Number

SKUGTIN
345810-250MGCN07790788049362

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