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Merck

IP05

Protein G Plus/Protein A Agarose Suspension

Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Synonym(s):

Affinity resin, Protein G/Agarose

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About This Item

NACRES:
NA.56
UNSPSC Code:
41116133

Key Documents

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form

slurry (Liquid)

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

technique(s)

protein purification: suitable

suitability

suitable for microbiology

shipped in

wet ice

storage temp.

2-8°C

Related Categories

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

Immunoprecipitation (see comments)

Disclaimer

Toxicity: Standard Handling (A)

General description

Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification.
Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Other Notes

Agarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.

Physical form

33% slurry in PBS.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

IP05-1.5ML:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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E Clark et al.
Journal of virology, 74(11), 5040-5052 (2000-05-09)
Productive high-titer infection by human immunodeficiency virus type 1 (HIV-1) requires the activation of target cells. Infection of quiescent peripheral CD4 lymphocytes by HIV-1 results in incomplete, labile reverse transcripts and lack of viral progeny formation. An interplay between Tat
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Oncology reports, 46(1) (2021-06-04)
Type 2 diabetes increases the risk various types of cancer and is associated with a poor prognosis therein. There is also evidence that the disease is associated with cancer metastasis. Centrosome amplification can initiate tumorigenesis with metastasis in vivo and increase
Chong Lian et al.
Aging, 12(22), 22599-22613 (2020-11-18)
In aging, the regulation of angiogenesis is a dynamic and complex process. We aimed to identify and characterize microRNAs that regulate angiogenesis during aging. We showed that, in response to vascular endothelial senescence, microRNA-25-3p (miR-25-3p) plays the role of an
Hailong Zhang et al.
Oncogene, 38(9), 1410-1431 (2018-10-12)
Argonaute2 (AGO2) is an effector of small RNA mediated gene silencing. Increasing evidence show that post-translational modifications of AGO2 can change miRNA activity at specific or global levels. Among the six mature miRNAs that are encoded by miR-17-92, miR-19b1 is
Priyanka Sharma et al.
Molecular cell, 73(1), 84-96 (2018-11-26)
The post-translational modification of key residues at the C-terminal domain of RNA polymerase II (RNAP2-CTD) coordinates transcription, splicing, and RNA processing by modulating its capacity to act as a landing platform for a variety of protein complexes. Here, we identify
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Agarose Beads for Immunoprecipitation and Antibody Purification

We manufacture IP and antibody purification agarose beads that purify immunoglobulins and IgG fractions. Our kits also provide a total antibody purification process

Global Trade Item Number

SKUGTIN
IP05-1.5MLCN04055977220223

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