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Merck

MAB331

Anti-SNAP-25 Antibody, clone SP14

ascites fluid, clone SP14, Chemicon®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
SP14, monoclonal
Species reactivity:
pig, human, hamster, rat
Application:
ELISA, IHC, WB
Citations:
35

Key Documents

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biological source

mouse

antibody form

ascites fluid

clone

SP14, monoclonal

species reactivity

pig, human, hamster, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

isotype

IgG1

NCBI accession no.

NM_003081.2, NM_130811.1

UniProt accession no.

P60880

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SNAP25(6616)

Immunogen

Crude synaptic immunoprecipitate (Human)

Application

Detect SNAP-25 using this Anti-SNAP-25 Antibody, clone SP14 validated for use in ELISA, WB, IH(P).
Immunohistochemistry at 1:500-1:5,000

Formalin fixed (<4 weeks), paraffin embedded

* It is suggested that you use an IgG specific second antibody to minimize possible non-specific staining.

Frozen

Western blotting at 1:100-1:1,000

ELISA at 1:500

It is reported that clone SP14 is capable of detecting SDS-resistant SNARE complexes {J. Cell Sci 2004 117(6):955-966}.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Synapse & Synaptic Biology

Biochem/physiol Actions

SNAP-25 (26-27 kDa). MAB331 is reactive with fusion protein from COS cells, but not with fusion protein from bacterial systems.

Physical form

Liquid. Contains no preservative.

Preparation Note

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

MAB331:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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High sensitivity of mouse neuronal cells to tetanus toxin requires a GPI-anchored protein.
Munro, P; Kojima, H; Dupont, JL; Bossu, JL; Poulain, B; Boquet, P
Biochemical and biophysical research communications null
Matthew J Van Hook et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(17), 4618-4634 (2017-04-02)
G-protein βγ subunits (Gβγ) interact with presynaptic proteins and regulate neurotransmitter release downstream of Ca2+ influx. To accomplish their roles in sensory signaling, photoreceptor synapses use specialized presynaptic proteins that support neurotransmission at active zone structures known as ribbons. While
Increased levels of SNAP-25 and synaptophysin in the dorsolateral prefrontal cortex in bipolar I disorder.
Scarr, E; Gray, L; Keriakous, D; Robinson, PJ; Dean, B
Bipolar Disorders null
Fatal familial insomnia with an unusual prion protein deposition pattern: an autopsy report with an experimental transmission study.
Sasaki, K; Doh-ura, K; Wakisaka, Y; Tomoda, H; Iwaki, T
Neuropathology and Applied Neurobiology null
Taste cells with synapses in rat circumvallate papillae display SNAP-25-like immunoreactivity.
Yang, R; Crowley, HH; Rock, ME; Kinnamon, JC
The Journal of Comparative Neurology null
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