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Merck

MABN15

Anti-Rhodopsin Antibody, clone 4D2

clone 4D2, from mouse

Synonym(s):

rhodopsin, rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant), retinitis pigmentosa 4, autosomal dominant, opsin 2, rod pigment

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
4D2, monoclonal
Application:
ELISA, ICC, IHC, WB
Citations:
38

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4D2, monoclonal

species reactivity

rat, human, fish

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_254276

UniProt accession no.

P51489

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... RHO(6010)
rat ... Rho(24717)

General description

Expected at ~ 39 kDa
Rhodopsin is a multi-pass, G-protein-coupled membrane receptor protein belonging to the G-protein coupled receptor 1 family. Found within rod photoreceptor cells, rhodopsin is activated by light photons in order for the visual transduction cascade to initialize. Rhodopsin is necessary to the forming of images in low light and for the viability of photoreceptor cells after birth. Mutations in rhodopsin expression are the most widely known cause of the human disease retinitis pigmentosa (RP) resulting in gradual loss of vision. At present, there are over 100 known rhodopsin mutations.

Immunogen

Epitope: N-terminus
Rat rod outer segments corresponding to rat Rhodopsin at the N-terminus.

Application

Anti-Rhodopsin Antibody, clone 4D2 is an antibody against Rhodopsin for use in IH, WB, IC, ELISA.
Research Category
Neuroscience
Research Sub Category
Sensory & PNS
Western Blot Analysis: A previous lot of this antibody detected Rhodopsin by WB in goldfish retina lysate, as reported by Knight K, et al.

Immunocytochemistry: A previous lot of this antibody detected Rhodopsin by ICC in goldfish retina tissue, as reported by Knight K, et al

Immunohistochemistry Analysis: 1:500 dilution of this antibody has been shown to detect Rhodopsin in human eye tissue.

ELISA: Specificity of this antibody, from a different lot, was validated in a competitive ELISA, as reported by Laird D, et al.

Biochem/physiol Actions

This antibody recognizes Rhodopsin at the N-terminus.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat eye tissue
Evaluated by Immunohistochemistry in rat eye tissue.

Immunohistochemistry Analysis: 1:500 dilution of this antibody detected Rhodopsin in rat eye tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

MABN15:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Zhe Zhang et al.
The Journal of biological chemistry, 295(34), 12153-12166 (2020-07-09)
Pheromones play critical roles in habitat identification and reproductive behavior synchronization in the sea lamprey (Petromyzon marinus). The bile acid 3-keto petromyzonol sulfate (3kPZS) is a major component of the sex pheromone mixture from male sea lamprey that induces specific
Liang Jia et al.
The Journal of biological chemistry, 297(5), 101268-101268 (2021-10-04)
Biogenic amines activate G-protein-coupled receptors (GPCRs) in the central nervous system in vertebrate animals. Several biogenic amines, when excreted, stimulate trace amine-associated receptors (TAARs), a group of GPCRs in the main olfactory epithelium, and elicit innate behaviors. How TAARs recognize
Kayvan Samimi et al.
Biomedical optics express, 13(6), 3476-3492 (2022-07-06)
Photoreceptors are the key functional cell types responsible for the initiation of vision in the retina. Phototransduction involves isomerization and conversion of vitamin A compounds, known as retinoids, and their recycling through the visual cycle. We demonstrate a functional readout
Devi Krishna Priya Karunakaran et al.
Investigative ophthalmology & visual science, 56(2), 787-798 (2015-01-17)
To understand how loss of citron kinase (CitK) affects retinal progenitor cells (RPCs) in the developing rat retina. We compared knockout (KO) and wild-type (WT) retinae by immunohistochemistry. The TdT-mediated dUTP terminal nick-end labeling (TUNEL) assay was performed to determine
Ashley A Rowe et al.
JCI insight, 6(17) (2021-07-23)
The metabolic environment is important for neuronal cells, such as photoreceptors. When photoreceptors undergo degeneration, as occurs during retinitis pigmentosa (RP), patients have progressive loss of vision that proceeds to full blindness. Currently, there are no available treatments for the
View All Related Papers

Global Trade Item Number

SKUGTIN
MABN1504053252518706

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