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Merck

PC205L

Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb

lyophilized, Calbiochem®

Synonym(s):

Anti-CGRP

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About This Item

NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
rat, mouse
Application:
—
Citations:
54

Key Documents

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biological source

rabbit

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized

contains

≤0.1% sodium azide as preservative

species reactivity

rat, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

dilution

(Free Floating Sections
Frozen Sections (1:2000-1:4000, ABC detection)
Immunofluorescence (1:100-1:200,
Paraffin Sections (s; heat pre-treatment required; ))

isotype

IgG

shipped in

ambient

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

mouse ... Calca(12310)

General description

Anti-CGRP, rabbit polyclonal, recognizes the α-CGRP in rat amygdala and spinal cord. It is validated for immunofluorescence, & for use with frozen, paraffin, and free-floating sections.
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the α-CGRP protein.
Recognizes the α-calcitonin gene related peptide in rat amygdala and spinal cord.

Immunogen

rat α-calcitonin gene related peptide (α-CGRP)

Application


Free Floating Sections (see application references)
Frozen Sections (1:2000-1:4000, ABC detection)
Immunofluorescence (1:100-1:200, see application references)
Paraffin Sections (see application references; heat pre-treatment required; see comments)

Preparation Note

Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Carefully reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Avoid freeze/thaw cycles of solutions.

Analysis Note

Positive Control
Rat amygdala or spinal cord

Other Notes

Antibody specificity was examined in the rat amygdala and spinal cord. Staining is completely eliminated by pre-treatment of antibody with 10-5M rat α-CGRP and partially eliminated by preabsorption with rat β-CGRP. Preabsorption with calcitonin resulted in no loss of immunostaining. For staining paraffin sections, heat sections to 80°C in 40 mM sodium citrate buffer, pH 8.5. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

US1PC205L-EACH: + PC205L-100UL: + PC205L-UL:

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Certificates of Analysis (COA)

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Min-Hee Oh et al.
Journal of immunology (Baltimore, Md. : 1950), 191(11), 5371-5382 (2013-10-22)
Chronic debilitating pruritus is a cardinal feature of atopic dermatitis (AD). Little is known about the underlying mechanisms. Antihistamines lack efficacy in treating itch in AD, suggesting the existence of histamine-independent itch pathways in AD. Transient receptor potential ankyrin 1
Stuart M Brierley et al.
Gastroenterology, 134(7), 2059-2069 (2008-03-18)
Although there are many candidates as molecular mechanotransducers, so far there has been no evidence for molecular specialization of visceral afferents. Here, we show that colonic afferents express a specific molecular transducer that underlies their specialized mechanosensory function: the transient
D J Van Daele et al.
Neuroscience, 162(2), 501-524 (2009-05-12)
The present study investigated the central connections of motor neurons innervating the thyroarytenoid laryngeal muscle that is active in swallowing, respiration and vocalization. In both intact and sympathectomized rats, the pseudorabies virus (PRV) was inoculated into the muscle. After initial
Liron S Duraku et al.
Molecular pain, 8, 61-61 (2012-09-01)
The epidermis is innervated by fine nerve endings that are important in mediating nociceptive stimuli. However, their precise role in neuropathic pain is still controversial. Here, we have studied the role of epidermal peptidergic nociceptive fibers that are located adjacent
Paula Dietrich et al.
Human molecular genetics, 21(23), 5078-5090 (2012-08-28)
Hereditary sensory and autonomic neuropathies (HSANs) encompass a group of genetically inherited disorders characterized by sensory and autonomic dysfunctions. Familial dysautonomia (FD), also known as HSAN type III, is an autosomal recessive disorder that affects 1/3600 live births in the
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Global Trade Item Number

SKUGTIN
PC205L-100ULCN04055977209532

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