IEVH85R
Immobilon® -E PVDF Membrane
1 roll, 8.5 cm × 10 m, 0.45 µm pore size, transfer membrane wets out in transfer buffer or water
Synonym(s):
Immobilon® NOW, Immobilon® narrow roll, Western blotting membrane, blotting membrane, transfer membrane
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About This Item
Product Name
Immobilon®-E PVDF Membrane, The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
material
PVDF membrane
plain filter
white filter
feature
easily wetted with water
manufacturer/tradename
Immobilon®
technique(s)
western blot: suitable
filter L × W
8.5 cm × 10 m
pore size
0.45 μm pore size
compatibility
for use with Amido black
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Transillumination
detection method
chemiluminescent
colorimetric
radioactive
shipped in
ambient
Quality Level
Related Categories
General description
Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most Western blotting applications, especially proteins greater than 20 kDa
- Compatible with chemiluminescent and chromogenic detection
Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes.
Features & Benefits:
- High protein binding provides stronger signal
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be stripped and reprobed multiple times
Legal Information
Storage Class
11 - Combustible Solids
wgk
WGK 3
Certificates of Analysis (COA)
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Articles
A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.
Understand the importance of using high-quality water in protein electrophoresis and Western blotting to improve assay sensitivity and accuracy.
Protocols
メルクのプレミックス溶液とプロトコルでSDS-PAGEゲル調製を簡素化し、正確なタンパク質分離を達成しましょう。
Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.
This page shows and discusses three protocols for stripping and reprobing a western blot membrane.
Related Content
We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.
我々はそれを理解している。ウエスタンはしみ抜きで苦労することがある。そこで、あなたの醜いブロットを私たちと共有し、サプライズを受け取ってください！それが何であるかは言えませんが-それは驚きを台無しにしてしまうからです-ラボの創造性を掻き立てるということを除いては。
Global Trade Item Number
| SKU | GTIN |
|---|---|
| IEVH85R | 04054839569098 |
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