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Merck

4B200

Sepharose™ 4B

45-165 μm bead diameter

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About This Item

CAS Number:
9012-36-6
UNSPSC Code:
12352200
PubChem Substance ID:
24873317
NACRES:
NA.21
MDL number:
MFCD08457579

Key Documents

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SMILES string

O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

biological source

algae (marine)

form

suspension

technique(s)

affinity chromatography: suitable

bead diameter

45-165 μm

pore size

30,000-50,000 Da fractionation range (Dextrans), 60,000-20,000,000 Da fractionation range (Globular proteins)

storage temp.

2-8°C

Quality Level

200

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General description

Sepharose™ 4B is an agarose bead and a polymer of Gal β1,4 [3,6]-anhydro-L-galactose. Sepharose aids in fractionating larger molecules and is devoid of charged saccharides. Presence of 3,6-anhydro-L-galactose resists biological degradation. Hydrogen bonds stabilize the sepharose gel structure. Sepharose is widely used in affinity chromatography by cross linking various ligands on the matrix.

Application

Sepharose™ 4B has been used:
  • to conjugate scorpion venom to be used in affinity chromatography
  • for clearing of protein extracts prior to immunoprecipitation
  • for gel purification and desalting of phospholipid vesicles
  • for adsorption of immunoglobulin G (IgG)

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

100.4 - 109.4 °F - closed cup

flash_point_c

38 - 43 °C - closed cup

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Group 4: Flammable liquids + Type 2 petroleums + Hazardous rank III + Water insoluble liquid

fsl

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

4B200-VAR: + 4B200-100ML:4548173936918 + 4B200-BULK: + 4B200-1L:4548173936925 + 4B200-1L-C: + 4B200-500ML:4548173936932 + 4B200-PM:

jan

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Certificates of Analysis (COA)

Lot/Batch Number
MKCZ9618
MKCZ8546
MKCZ3155
MKCX1071
MKCZ1415

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Construction of a liposome dialyzer for the preparation of high-value, small-volume liposome formulations
Adamala K, et al.
Nature Protocols, 10(6), 927-927 (2015)
Oral Absorption of Mesobuthus eupeus Scorpion Venom in Mice.
Hosseini Z, et al.
Iranian Journal of Toxicology, 11(2), 15-19 (2017)
Principles and Reactions of Protein Extraction, Purification, and Characterization (2004)
Paul Kaufmann et al.
PloS one, 14(8), e0220866-e0220866 (2019-08-08)
Dipeptidyl amino-peptidase 3 (DPP3) is an aminopeptidase involved in peptide degradation, including hormone peptides as angiotensin II and enkephalins. DPP3 plasma activity increases in septic patients and correlates with mortality risk. However, the exact physiological role of DPP3 remains unclear
Mouse Rif1 is a regulatory subunit of protein phosphatase 1 (PP1)
Sukackaite R, et al.
Scientific Reports, 7(1), 2119-2119 (2017)
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