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P7170

Ponceau S solution

BioReagent, 0.1 % (w/v) in 5% acetic acid

Synonym(s):

Ponceau S

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About This Item

Empirical Formula (Hill Notation):
C22H12N4Na4O13S4
CAS Number:
6226-79-5
Molecular Weight:
760.57
UNSPSC Code:
12171500
PubChem Substance ID:
24898827
NACRES:
NA.47
MDL number:
MFCD00003892
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Product Name

Ponceau S solution, BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid

description

for protein analysis

Quality Segment

200

product line

BioReagent

form

liquid

concentration

0.1 % (w/v) in 5% acetic acid

technique(s)

western blot: suitable

density

1.01 g/cm3 at 20 °C

suitability

suitable (for use in cellulose acetate electrophoresis), suitable for electrophoresis

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;

InChI key

KQHKSGRIBYJYFX-WNDMICSESA-J

General description

Ponceau S is the sodium salt of a diazo dye. Ponceau S solution is a suitable reagent for use in electrophoresis studies. The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1M NaOH for 1 minute. The stained and destained membrane can then be immunologically detected (Western blotting). A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.

Application

Ponceau S solution has been used for membrane staining (Western blotting).

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

P7170-30ML:4548173951171 + P7170-BULK: + P7170-1L:4548173951164 + P7170-VAR:

jan

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