Skip to Content
Merck

P7170

Ponceau S solution

BioReagent, 0.1 % (w/v) in 5% acetic acid

Synonym(s):

Ponceau S

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

Empirical Formula (Hill Notation):
C22H12N4Na4O13S4
CAS Number:
6226-79-5
Molecular Weight:
760.57
UNSPSC Code:
12171500
PubChem Substance ID:
24898827
NACRES:
NA.47
MDL number:
MFCD00003892

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Ponceau S solution, BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid

SMILES string

[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O

InChI key

KQHKSGRIBYJYFX-WNDMICSESA-J

InChI

1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;

description

for protein analysis

product line

BioReagent

form

liquid

concentration

0.1 % (w/v) in 5% acetic acid

technique(s)

western blot: suitable

density

1.01 g/cm3 at 20 °C

suitability

suitable (for use in cellulose acetate electrophoresis), suitable for electrophoresis

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Ponceau S is the sodium salt of a diazo dye. Ponceau S solution is a suitable reagent for use in electrophoresis studies. The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1M NaOH for 1 minute. The stained and destained membrane can then be immunologically detected (Western blotting). A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.

Application

Ponceau S solution has been used for membrane staining (Western blotting).

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

P7170-30ML:4548173951171 + P7170-BULK: + P7170-1L:4548173951164 + P7170-VAR:

jan

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000426978
0000426978
0000427010
0000427010
0000316702

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Immunomodulatory effect of arsenic on cytokine and HSP gene
expression in Labeo rohita fingerlings
Sudeshna Banerjee
Fish & Shellfish Immunology, 44 (2015)
Joshua P Nederveen et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(7), 8975-8989 (2020-05-29)
Skeletal muscle satellite cell (SC) function and responsiveness is regulated, in part, through interactions within the niche, in which they reside. Evidence suggests that structural changes occur in the SC niche as a function of aging. In the present study
Legume Grains Enhance Ileal Losses of Specific Endogenous Serine-Protease Proteins in Weaned Pigs1
Paulo Salgado
The Journal of Nutrition (2002)
Challa S.S.R. Kumar
Semiconductor Nanomaterials (2010)
Shankar Suman et al.
PLoS neglected tropical diseases, 12(9), e0006792-e0006792 (2018-09-18)
The protozoan parasite Trypanosoma cruzi, the causative agent of Chagas disease, causes severe morbidity and mortality in afflicted individuals. About 30% of T. cruzi-infected individuals present with cardiac, gastrointestinal tract, and/or neurological disorders. Megacolon, one of the major pathologies of
View All Related Papers

Articles

タンパク質染色試薬選択ガイド

Sigmaでは、タンパク質可視化のためのEZBlue™ 試薬とProteoSilver™ 試薬をご提供しています。プロテオミクスや従来のPAGEフォーマットに適しています。

Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Protocols

ウェスタンブロッティングプロトコル － イムノブロッティングまたはウェスタンブロット

ウェスタンブロッティングプロトコルでは、研究におけるイムノブロッティング手順で極めて重要なゲルからニトロセルロースへのタンパク質転写を詳述します。

Western Blot Protocol - Immunoblotting or Western Blot

Western blot protocol details protein transfer from gels to nitrocellulose, crucial for immunoblotting procedures in research.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service