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RDD035

HEPES sodium salt

≥99.5% (titration), free-flowing, Redi-Dri™

Synonym(s):

HEPES Sodium, HEPES hemisodium salt, Sodium HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt

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About This Item

Empirical Formula (Hill Notation):
C8H17N2NaO4S
CAS Number:
75277-39-3
Molecular Weight:
260.29
UNSPSC Code:
12161700
NACRES:
NA.25
MDL number:
MFCD00036463
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product line

Redi-Dri™

Quality Level

100

assay

≥99.5% (titration)

quality

free-flowing

useful pH range

6.8-8.2

pKa (25 °C)

7.5

application(s)

diagnostic assay manufacturing

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

General description

HEPES sodium salt is essential to prepare HEPES buffer that is commonly used for cell culture and protein applications. It is useful for maintaining homeostasis when working with cells outside of the incubator by balancing CO2. HEPES buffering capacity exceeds most buffers, with a pKa of 7.5, making it optimal for maintaining pH in biological systems. In solution, it is non-toxic, zwitterion with a physiological buffering range of pH 6.8 - 8.2. It has a low metal binding capacity and is safe to use in research for metal-binding proteins.

Application

HEPES sodium salt can be used:
  • as a protein precipitation buffer
  • in cell culture media
  • as a binding buffer

Features and Benefits

  • Redi-Dri™ eliminates clumping for hygroscopic chemicals
  • powder remains free-flowing and easy to use
  • Safer to handle and weigh out for standard use in lab
  • Eliminates clumps, maintains product moisture, quality, and characteristics

Physical form

Lyophilized, spray dried, or crystallized material.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

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Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

RDD035-500G: + RDD035-1KG: + RDD035-2.5KG: + RDD035-BULK: + RDD035-VAR: + RDD035-100G:

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Certificates of Analysis (COA)

Lot/Batch Number
0000545515
0000506533
0000314581
SLCF3906

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Articles

Redi-Dri™ – The World of Free-flowing Hygroscopic Products

Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.

Nishant Mehta et al.
Cell reports, 28(10), 2509-2516 (2019-09-05)
V-domain immunoglobulin (Ig) suppressor of T cell activation (VISTA) is an immune checkpoint protein that inhibits the T cell response against cancer. Similar to PD-1 and CTLA-4, a blockade of VISTA promotes tumor clearance by the immune system. Here, we report a
Åsa Birna Birgisdottir et al.
Autophagy, 15(8), 1333-1355 (2019-02-16)
Autophagosome formation depends on a carefully orchestrated interplay between membrane-associated protein complexes. Initiation of macroautophagy/autophagy is mediated by the ULK1 (unc-51 like autophagy activating kinase 1) protein kinase complex and the autophagy-specific class III phosphatidylinositol 3-kinase complex I (PtdIns3K-C1). The
Peng Yang
Macromolecular bioscience, 12(8), 1053-1059 (2012-06-19)
When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001-0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue"
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