46955
Fluorescein
suitable for fluorescence, free acid
Synonym(s):
Acid Yellow 73
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About This Item
Product Name
Fluorescein, suitable for fluorescence, free acid
InChI key
GNBHRKFJIUUOQI-UHFFFAOYSA-N
InChI
1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1
Quality Level
Gene Information
human ... PTPN1(5770), PTPRF(5792)
assay
>95.0% (HPLC)
form
powder or crystals
mol wt
332.31 g/mol
concentration
≤100% (2-(6-hydroxy-3-oxo-(3H)-xanthen-9-yl)benzoic acid)
technique(s)
titration: suitable
color
red
mp
320 °C
solubility
methanol: 0.1 g/10 mL, orange to very deep orange
λmax
486.0-501.0 nm
fluorescence
λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0
suitability
suitable for fluorescence
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Application
- in the permeability assay of Caco-2 cells
- to test its suitability to serve as lifetime fluorescence standards
- as a fluorophore for the fast recovery of fluorescence peaks and fluorophore concentration in the presence of hemoglobin
- as a fluorescent probe in oxygen radical absorbance capacity (ORAC) assay
General description
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Articles
Fluorescence quenching microscopy visualizes 2D materials like graphene and MoS2 rapidly, inexpensively, and with high fidelity.
蛍光寿命測定には、蛍光強度に基づく測定法にない利点があります。応用例として、蛍光寿命分析、蛍光寿命センシングおよび蛍光寿命イメージング（FLI）が挙げられます。
蛍光消光顕微鏡は、グラフェンやMoS2のような2次元材料を、迅速かつ安価に、高い忠実度で可視化する。
Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.
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