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Merck

85878

Streptavidin from Streptomyces avidinii

affinity purified, lyophilized from 10 mM potassium phosphate, ≥13 U/mg protein

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About This Item

CAS Number:
9013-20-1
UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
MFCD00082035
Form:
powder
Mol wt:
Mr ~60000

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form

powder

quality

affinity purified, lyophilized from 10 mM potassium phosphate

specific activity

≥13 U/mg protein

mol wt

Mr ~60000

solubility

H2O: 1 mg/mL, clear to hazy, colorless to faintly yellow

storage temp.

−20°C

Quality Level

200

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General description

Streptavidin is a crystalline protein synthesized by Streptomycetes.

Application

Streptavidin from Streptomyces avidinii has been used:
  • in the pre-functionalization of self-propelled catalytic micromotors
  • in the fabrication and modification of hydroxyapatite-chitosan (HA-CTS) nanofilm-coated substrates
  • as an analyte to bind biomolecules to the gold-inverted polymer solar cells (Au-IPSC)

Biochem/physiol Actions

Streptavidin is a biotin binding protein and has the ability to bind four molecules of biotin. The biotin binding pocket of streptavidin helps to interact with biotin. It can be used to enhance protein binding and multimerization.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.
The sales quantitites 1 mg and 5 mg

Analysis Note

Binding capability: Streptavidin binds one molecule of biotin per subunit. Comparison of biotin binding and absorbance measurements indicates that ≤5% binding sites are occupied.

Other Notes

1 U corresponds to the amount of protein which binds 1μg (+)-biotin at pH 7.5
Review: Application of the avidin-biotin technique in microbiology; Protein isolated from the bacterium Streptomyces avidinii, having a high affinity for biotin; Avidin-Biotin technology

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

85878-VAR: + 85878-5MG: + 85878-BULK: + 85878-1MG-PW: + 85878-10MG: + 85878-10MG-PW: + 85878-1MG: + 85878-5MG-PW:

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Certificates of Analysis (COA)

Lot/Batch Number
0000334443
0000334443
0000302572
0000403979
0000453596

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D.A. Fuccillo
Biotechniques, 3, 494-494 (1985)
Soon Gang Choi et al.
Nature communications, 10(1), 3907-3907 (2019-08-31)
Complementary assays are required to comprehensively map complex biological entities such as genomes, proteomes and interactome networks. However, how various assays can be optimally combined to approach completeness while maintaining high precision often remains unclear. Here, we propose a framework
Scientific and Clinical Applications of Magnetic Carriers (2013)
Capture and identification of heterogeneous circulating tumor cells using transparent nanomaterials and quantum dots-based multiplexed imaging
Chen Y Y, et al.
Journal of Cancer, 7(1), 69-69 (2016)
M. Wilchek and E.A. Bayer, eds.
Methods of Enzymatic Analysis, 184 (1990)
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